Zhang Mengying, Zhang Wenyan, Zheng Zijian, Zhang Zhiping, Hua Bing, Liu Jiexia, Miao Minmin
College of Horticulture and Landscape, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Plants (Basel). 2023 Mar 9;12(6):1252. doi: 10.3390/plants12061252.
The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family (NPF) proteins perform an essential role in regulating plant nitrate absorption and distribution and in improving plant nitrogen use efficiency. In this study, cucumber ( L.) genes were comprehensively analyzed at the whole genome level, and 54 genes were found to be unevenly distributed on seven chromosomes in the cucumber genome. The phylogenetic analysis showed that these genes could be divided into eight subfamilies. We renamed all genes according to the international nomenclature, based on their homology with genes. By surveying the expression profiles of genes in various tissues, we found that was specifically expressed in roots, indicating that may play a role in N absorption; was highly expressed in petioles, which may be related to NO storage in petioles; and was highly expressed in fruits, which may promote NO transport to the embryos. We further examined their expression patterns under different abiotic stress and nitrogen conditions, and found that and responded to salt, cold, and low nitrogen stress. Taken together, our study lays a foundation for further exploration of the molecular and physiological functions of cucumber nitrate transporters.
硝酸盐转运蛋白1/肽转运蛋白家族（NPF）蛋白在调节植物硝酸盐吸收与分配以及提高植物氮利用效率方面发挥着重要作用。在本研究中，对黄瓜（L.）基因进行了全基因组水平的综合分析，发现54个基因在黄瓜基因组的7条染色体上分布不均。系统发育分析表明，这些基因可分为8个亚家族。我们根据与基因的同源性，按照国际命名法对所有基因进行了重新命名。通过调查基因在不同组织中的表达谱，我们发现基因在根中特异性表达，表明其可能在氮吸收中发挥作用；基因在叶柄中高表达，这可能与叶柄中硝酸盐的储存有关；基因在果实中高表达，这可能促进硝酸盐向胚的转运。我们进一步研究了它们在不同非生物胁迫和氮条件下的表达模式，发现基因和对盐、冷和低氮胁迫有响应。综上所述，我们的研究为进一步探索黄瓜硝酸盐转运蛋白的分子和生理功能奠定了基础。
