Huang Zhen, Zhang Xuexian, Jiang Shouhua, Qin Mengfan, Zhao Na, Lang Lina, Liu Yaping, Tian Zhengshu, Liu Xia, Wang Yang, Zhang Binbin, Xu Aixia
State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Huai an Academy of Agricultural Sciences, Huai an, Jiangsu, 223001, China.
Breed Sci. 2017 Jun;67(3):213-220. doi: 10.1270/jsbbs.16161. Epub 2017 May 23.
Currently, cold temperatures are one of the main factors threatening rapeseed production worldwide; thus, it is imperative to identify cold-resistant germplasm and to cultivate cold-resistant rapeseed varieties. In this study, the cold resistance of four varieties was analyzed. The cold resistance of Longyou6 and Longyou7 was better than that of Tianyou2 and Tianyou4. Thus, an F population derived from Longyou6 and Tianyou4 was used to study the correlation of cold resistance and physiological indexes. Our results showed that the degree of frost damage was related to the relative conductivity and MDA content (r1 = 0.558 and r2 = 0.447, respectively). In order to identify the markers related to cold resistance, 504 pairs of SSR (simple sequence repeats) primers were used to screen the two parents and F population. Four and five SSR markers had highly significant positive correlation to relative conductivity and MDA, respectively. In addition, three of these SSR markers had a highly significant positive correlation to both of these two indexes. These three SSR markers were subsequently confirmed to be used to distinguish between cold-resistant and non-cold-resistant varieties. The results of this study will lay a solid foundation for the mapping of cold-resistant genes and molecular markers assisted selection for the cold-resistance.
目前，低温是威胁全球油菜籽生产的主要因素之一；因此，鉴定抗寒种质并培育抗寒油菜品种势在必行。在本研究中，分析了四个品种的抗寒性。龙油6号和龙油7号的抗寒性优于天油2号和天油4号。因此，以龙油6号和天油4号构建的F群体为材料，研究抗寒性与生理指标的相关性。我们的结果表明，冻害程度与相对电导率和丙二醛含量相关（相关系数r1分别为0.558，r2分别为0.447）。为了鉴定与抗寒相关的标记，使用504对SSR（简单序列重复）引物对两个亲本和F群体进行筛选。分别有4个和5个SSR标记与相对电导率和丙二醛呈极显著正相关。此外，其中3个SSR标记与这两个指标均呈极显著正相关。随后证实这3个SSR标记可用于区分抗寒和不抗寒品种。本研究结果将为抗寒基因定位及抗寒分子标记辅助选择奠定坚实基础。
