Salouti Mojtaba, Saghatchi Faranak
Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Department of Radiology, Faculty of Paramedical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran.
IET Nanobiotechnol. 2017 Aug;11(5):604-611. doi: 10.1049/iet-nbt.2016.0191.
Using of targeted contrast agents in X-ray imaging of breast cancer can improve the accuracy of diagnosis, staging, and treatment planning by providing early detection and superior definition of tumour volume. This study demonstrates a new class of X-ray contrast agents based on gold nanoparticles (GNPs) and bombesin (BBN) for imaging of breast cancer in radiology. GNPs were synthesised in spherical shape in the size range of 15 ± 2 nm and conjugated with BBN followed by coating with polyethyleneglycol (PEG). The and behaviour of PEG-coated GNPs-BBN conjugate was investigated performing cytotoxicity, binding, and internalisation assays as well as biodistribution and X-ray imaging studies in mouse bearing breast tumour. Cytotoxicity study showed biocompatibility of the prepared bioconjugate. The binding and internalisation studies using T47D cell line approved the targeting ability of new agent. The biodistribution study showed the considerable accumulation of prepared conjugate in breast tumour in mouse model. The breast tumour was clearly visualised in X-ray images taken from the mouse model. The results showed the potential of PEG-coated GNPs-BBN conjugate as a contrast agent in X-ray imaging of breast tumour in humans that need further investigations.
在乳腺癌的X射线成像中使用靶向造影剂，通过提供肿瘤体积的早期检测和更高清晰度，可提高诊断、分期及治疗规划的准确性。本研究展示了一类基于金纳米颗粒（GNP）和蛙皮素（BBN）的新型X射线造影剂，用于放射学中乳腺癌的成像。GNP被合成成尺寸范围为15±2 nm的球形，并与BBN偶联，随后用聚乙二醇（PEG）包被。通过在荷乳腺肿瘤小鼠中进行细胞毒性、结合、内化测定以及生物分布和X射线成像研究，对PEG包被的GNP-BBN偶联物的行为进行了研究。细胞毒性研究表明所制备的生物偶联物具有生物相容性。使用T47D细胞系进行的结合和内化研究证实了新型试剂的靶向能力。生物分布研究表明所制备的偶联物在小鼠模型的乳腺肿瘤中有大量蓄积。从小鼠模型拍摄的X射线图像中可清晰看到乳腺肿瘤。结果表明，PEG包被的GNP-BBN偶联物有潜力作为一种造影剂用于人类乳腺肿瘤的X射线成像，但还需要进一步研究。
