Rathbun Colin M, Prescher Jennifer A
Department of Chemistry, ‡Department of Molecular Biology and Biochemistry, and §Department of Pharmaceutical Sciences, University of California , Irvine, California 92697, United States.
Biochemistry. 2017 Oct 3;56(39):5178-5184. doi: 10.1021/acs.biochem.7b00435. Epub 2017 Jul 26.
Bioluminescence with luciferase-luciferin pairs is an attractive method for surveying cells in live tissues and whole organisms. Recent advances in luciferin chemistry and luciferase engineering are further expanding the scope of the technology. It is now possible to spy on cells in a variety of deep tissues and visualize multicellular interactions, feats that are enabling new questions to be asked and new ideas to be explored. This perspective piece highlights recent successes in bioluminescent probe development and their applications to imaging in live cells, tissues, and animals.
荧光素酶 - 荧光素对的生物发光是一种用于监测活组织和整个生物体中细胞的有吸引力的方法。荧光素化学和荧光素酶工程的最新进展进一步扩大了该技术的范围。现在可以窥探各种深部组织中的细胞并可视化多细胞相互作用，这些成果使得人们能够提出新问题并探索新想法。这篇观点文章重点介绍了生物发光探针开发的最新成功及其在活细胞、组织和动物成像中的应用。
