State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Nutrients. 2018 Aug 16;10(8):1105. doi: 10.3390/nu10081105.
Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut. Previous studies focused on the growth stimulation of , but they did not further investigate the bifidobacterial composition and the specific species that were stimulated. In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular , was analyzed by sequencing the V3⁻V4 region and the gene on the MiSeq platform, respectively. In the high-dose group, the relative abundance of Actinobacteria was significantly increased, which was mainly contributed by . At the genus level, the relative abundances of and were also significantly increased. Through the sequencing, 14 species of were identified, among which was most abundant. After FOS treatment, became almost the sole bifidobacterial species (>95%). strains were isolated and demonstrated their ability to metabolize FOS by high performance liquid chromatography (HPLC). Therefore, we inferred that FOS significantly stimulated the growth of in mice. Further investigations are needed to reveal the mechanism of selectiveness between FOS and , which would aid our understanding of the basic principles between dietary carbohydrates and host health.
果寡糖（FOS）是一种众所周知的益生元，被认为能选择性地刺激肠道中双歧杆菌的生长。先前的研究主要集中在生长刺激上，但没有进一步研究双歧杆菌的组成和被刺激的特定物种。在这项研究中，用不同剂量的 FOS 喂养小鼠四周，然后分别通过 MiSeq 平台上的 V3-V4 区和 16S rRNA 基因测序分析粪便微生物群的组成。在高剂量组中，放线菌的相对丰度显著增加，主要由 贡献。在属水平上， 和 的相对丰度也显著增加。通过 16S rRNA 测序，共鉴定出 14 种 ，其中 最为丰富。经 FOS 处理后， 几乎成为唯一的双歧杆菌物种（>95%）。分离出 菌株，并通过高效液相色谱（HPLC）证明了它们代谢 FOS 的能力。因此，我们推断 FOS 显著刺激了小鼠中 的生长。需要进一步的研究来揭示 FOS 与 之间选择性的机制，这将有助于我们理解膳食碳水化合物与宿主健康之间的基本原理。
