Guo Jialiang, Dong Weichong, Jin Lin, Wang Pengcheng, Hou Zhiyong, Zhang Yingze
Department of Orthopaedic Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.
Key Laboratory of Orthopaedic Biomechanics of Hebei Province, Shijiazhuang, Hebei, People's Republic of China.
Int Orthop. 2017 Oct;41(10):2119-2128. doi: 10.1007/s00264-017-3581-4. Epub 2017 Jul 26.
As an antioxidant molecule, hydrogen has been received much more attention and reported to be used as the treatment strategy for various diseases. In this study, we hypothesize that systemic delivery of hydrogen saline water may improve the reservation of bone tissue in the tibias and femurs of osteoporotic rats caused by diabetes mellitus (DM), which is characterized by increased levels of oxidative stress and overproducing reactive oxygen species (ROS).
The animals were divided into three groups of 12 animals and lavaged with normal saline (normal control and DM), or hydrogen saline water (DM + HRS). General status, blood glucose level, tibial and femoral mechanical strength, and micro-CT scans of the proximal tibia were recorded and analyzed.
After 12 weeks, the glucose level was significantly decreased in the DM + HRS group compared with that of the DM group. Micro-CT scans showed that bone volume/total volume, connectivity density, trabecular thickness, and trabecular number were significantly increased compared with the DM group. Mechanical results of energy, stiffness and elastic modulus in the DM + HRS group were significantly higher than in the other groups for the tibia and femur.
The results indicate that the systemic delivery of hydrogen saline water, which is safe and well tolerated, preserves bone volume and decreases fracture risks in streptozotocin-induced diabetic status rats, whose bone structure or inherent material properties of bone tissues are changed.
作为一种抗氧化分子，氢气已受到更多关注，并被报道可作为多种疾病的治疗策略。在本研究中，我们假设全身给予富氢盐水可能会改善糖尿病（DM）所致骨质疏松大鼠胫骨和股骨的骨组织保留情况，糖尿病的特征是氧化应激水平升高和活性氧（ROS）过度产生。
将动物分为三组，每组12只，分别用生理盐水灌胃（正常对照组和糖尿病组）或富氢盐水灌胃（糖尿病 + 富氢盐水组）。记录并分析动物的一般状况、血糖水平、胫骨和股骨的力学强度以及胫骨近端的显微CT扫描结果。
12周后，糖尿病 + 富氢盐水组的血糖水平与糖尿病组相比显著降低。显微CT扫描显示，与糖尿病组相比，骨体积/总体积、连接密度、骨小梁厚度和骨小梁数量均显著增加。糖尿病 + 富氢盐水组胫骨和股骨的能量、刚度和弹性模量的力学结果显著高于其他组。
结果表明，全身给予富氢盐水安全且耐受性良好，可保留骨体积并降低链脲佐菌素诱导的糖尿病状态大鼠的骨折风险，这些大鼠的骨组织结构或骨组织的固有材料特性发生了改变。
