恒河猴1型单纯疱疹病毒生殖器感染模型。
A model of genital herpes simplex virus Type 1 infection in Rhesus Macaques.
作者信息
Aravantinou Meropi, Frank Ines, Arrode-Bruses Geraldine, Szpara Moriah, Grasperge Brooke, Blanchard James, Gettie Agegnehu, Derby Nina, Martinelli Elena
机构信息
Center for Biomedical Research, Population Council, New York, NY, USA.
The Pennsylvania State University, University Park, PA, USA.
出版信息
J Med Primatol. 2017 Aug;46(4):121-128. doi: 10.1111/jmp.12293.
BACKGROUND
Although HSV-2 is the major cause of genital lesions, HSV-1 accounts for half of new cases in developed countries.
METHODS
Three healthy SHIV-SF162P3-infected Indian rhesus macaques were inoculated with 4×10 pfu of HSV-1 twice, with the second inoculation performed after the vaginal mucosa was gently abraded with a cytobrush.
RESULTS
HSV-1 DNA was detected in vaginal swabs 5 days after the second but not the first inoculation in all three macaques. An increase in inflammatory cytokines was detected in the vaginal fluids of the animals with no or intermittent shedding. Higher frequency of blood α β CD4 T cells was measured in the animals with consistent and intermitted shedding, while a decrease in the frequency of CD69 CD4 T cells was present in all animals.
CONCLUSIONS
This macaque model of genital HSV-1 could be useful to study the impact of the growing epidemic of genital HSV-1 on HIV infection.
背景
虽然单纯疱疹病毒2型（HSV - 2）是生殖器病变的主要病因，但在发达国家，HSV - 1导致的新发病例占一半。
方法
三只感染了健康的猴 - 人免疫缺陷病毒（SHIV） - SF162P3的印度恒河猴，两次接种4×10个噬斑形成单位（pfu）的HSV - 1，第二次接种是在使用细胞刷轻轻擦伤阴道黏膜后进行。
结果
在所有三只猕猴中，第二次接种后5天在阴道拭子中检测到HSV - 1 DNA，而第一次接种后未检测到。在无脱落或间歇性脱落的动物的阴道分泌物中检测到炎性细胞因子增加。在持续和间歇性脱落的动物中测量到血液αβCD4 T细胞频率更高，而所有动物中CD69 CD4 T细胞频率均降低。
结论
这种生殖器HSV - 1猕猴模型可能有助于研究生殖器HSV - 1流行增加对HIV感染的影响。
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