Jin L, Xu S, Maple P A C, Xu W, Brown K E
Virus Reference Department,National Infections Service,Public Health England,London,UK.
Institute of Viral Disease Control and Prevention,Chinese Centers for Disease Control and Prevention,Beijing,China.
Epidemiol Infect. 2017 Sep;145(12):2618-2625. doi: 10.1017/S0950268817001509. Epub 2017 Jul 27.
Varicella-zoster virus (VZV) infection (chickenpox) results in latency and subsequent reactivation manifests as shingles. Effective attenuated vaccines (vOka) are available for prevention of both illnesses. In this study, an amplicon-based sequencing method capable of differentiating between VZV wild-type (wt) strains and vOka vaccine is described. A total of 44 vesicular fluid specimens collected from 43 patients (16 from China and 27 from the UK) with either chickenpox or shingles were investigated, of which 10 had received previous vaccination. Four sets of polymerase chain reactions were set up simultaneously with primers amplifying regions encompassing four single nucleotide polymorphisms (SNPs), '69349-106262-107252-108111'. Nucleotide sequences were generated by Sanger sequencing. All samples except one had a wt SNP profile of 'A-T-T-T'. The sample collected from a patient who received vaccine 7-10 days ago, along with VZV vaccine preparations, Zostavax and Baike-varicella gave a SNP profile 'G-C-C-C'. The results show that this method can distinguish vaccine-derived virus from wt viruses from main four clades, (clades 1-4) and should be of utility worldwide.
水痘带状疱疹病毒（VZV）感染（水痘）会导致潜伏，随后病毒重新激活会表现为带状疱疹。有效的减毒疫苗（vOka）可用于预防这两种疾病。在本研究中，描述了一种基于扩增子的测序方法，该方法能够区分VZV野生型（wt）毒株和vOka疫苗。共对从43例水痘或带状疱疹患者（16例来自中国，27例来自英国）收集的44份水疱液标本进行了研究，其中10例曾接种过疫苗。同时设置了四组聚合酶链反应，引物扩增包含四个单核苷酸多态性（SNP）的区域，即“69349-106262-107252-108111”。通过桑格测序生成核苷酸序列。除一份样本外，所有样本的wt SNP谱均为“A-T-T-T”。从一名在7-10天前接种疫苗的患者身上采集的样本，连同VZV疫苗制剂Zostavax和百克-水痘，其SNP谱为“G-C-C-C”。结果表明，该方法能够区分来自四个主要分支（分支1-4）的疫苗衍生病毒和wt病毒，在全球范围内应具有实用性。
