Cao Nan, Li Wanyan, Li Bingxin, Tian Yunbo, Xu Danning
Institute of Animal Nutrition, Genetics and Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Anim Sci J. 2017 Dec;88(12):2001-2009. doi: 10.1111/asj.12861. Epub 2017 Jul 27.
The goose is an economically important poultry species and a principal natural host of avian viruses. This study aimed to determine the effects of selenium on the immune response of geese. Under selenium stimulation, gene expression profiling was investigated using transcriptome sequencing. The selenoproteins were promoted by selenium stimulation, while the heat shock proteins, interleukin and interferons were mainly down-regulated. After comparison, 2228 differentially expressed genes were primarily involved in immune and environmental response, and infectious disease and genetic information processing related pathways were identified. Specifically, the enzymes of the lysosomes which acted as a safeguard in preventing pathogens were mostly up-regulated and six randomly selected differentially expressed genes were validated by quantitative polymerase chain reaction. In addition, the most proportional increased transcription factor family basic helix-loop-helix (bHLH) located in the 5' flank of selenoprotein P-like protein for selenium metabolism was identified by response to the selenium stimulation in this study. These analyses show that selenium can promote immune function by activating selenoproteins, transcript factors and lysosome pathway related genes, while weakening cytokine content genes in geese.
鹅是一种具有重要经济价值的家禽品种，也是禽病毒的主要自然宿主。本研究旨在确定硒对鹅免疫反应的影响。在硒刺激下，利用转录组测序研究基因表达谱。硒刺激促进了硒蛋白的表达，而热休克蛋白、白细胞介素和干扰素主要下调。经比较，2228个差异表达基因主要参与免疫和环境反应，并鉴定出与传染病和遗传信息处理相关的途径。具体而言，作为预防病原体的保障的溶酶体酶大多上调，通过定量聚合酶链反应验证了6个随机选择的差异表达基因。此外，本研究通过对硒刺激的反应，鉴定出位于类硒蛋白P样蛋白5'侧翼的比例增加最多的转录因子家族碱性螺旋-环-螺旋（bHLH），其参与硒代谢。这些分析表明，硒可以通过激活硒蛋白、转录因子和溶酶体途径相关基因来促进鹅的免疫功能，同时减弱鹅体内细胞因子含量相关基因的表达。
