Research Laboratory of Electronics, Massachusetts Institute of Technology, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, MA 02139, USA.
Research Laboratory of Electronics, Massachusetts Institute of Technology, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, MA 02139, USA; Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA 02114, USA.
Curr Opin Biotechnol. 2017 Oct;47:133-141. doi: 10.1016/j.copbio.2017.06.011. Epub 2017 Aug 17.
Synthetic biology aims to introduce new sense-and-respond capabilities into living cells, which would enable novel therapeutic strategies. The development of regulatory elements, molecular computing devices, and effector screening technologies has enabled researchers to design synthetic gene circuits in many organisms, including mammalian cells. Engineered gene networks, such as closed-loop circuits or Boolean logic gate circuits, can be used to program cells to perform specific functions with spatiotemporal control and restoration of homeostasis in response to the extracellular environment and intracellular signaling. In addition, genetically modified microbes can be designed as local delivery of therapeutic molecules. In this review, we will discuss recent advances in therapeutic applications of synthetic gene circuits, as well as challenges and future opportunities for biomedicine.
合成生物学旨在为活细胞引入新的感知和响应能力，从而能够实现新的治疗策略。调控元件、分子计算设备和效应子筛选技术的发展使研究人员能够在许多生物体中设计合成基因回路，包括哺乳动物细胞。工程基因网络，如闭环电路或布尔逻辑门电路，可用于编程细胞，以时空控制执行特定功能，并在响应细胞外环境和细胞内信号时恢复体内平衡。此外，经过基因改造的微生物可以设计为局部递送达治疗分子。在这篇综述中，我们将讨论合成基因回路在治疗应用方面的最新进展，以及生物医学面临的挑战和未来机遇。
