Kis Zoltán, Pereira Hugo Sant'Ana, Homma Takayuki, Pedrigi Ryan M, Krams Rob
Department of Bioengineering, Imperial College London, London, UK.
Department of Bioengineering, Imperial College London, London, UK
J R Soc Interface. 2015 May 6;12(106). doi: 10.1098/rsif.2014.1000.
In this review, we discuss new emerging medical applications of the rapidly evolving field of mammalian synthetic biology. We start with simple mammalian synthetic biological components and move towards more complex and therapy-oriented gene circuits. A comprehensive list of ON-OFF switches, categorized into transcriptional, post-transcriptional, translational and post-translational, is presented in the first sections. Subsequently, Boolean logic gates, synthetic mammalian oscillators and toggle switches will be described. Several synthetic gene networks are further reviewed in the medical applications section, including cancer therapy gene circuits, immuno-regulatory networks, among others. The final sections focus on the applicability of synthetic gene networks to drug discovery, drug delivery, receptor-activating gene circuits and mammalian biomanufacturing processes.
在本综述中，我们讨论了哺乳动物合成生物学这一快速发展领域新出现的医学应用。我们从简单的哺乳动物合成生物学组件入手，进而探讨更复杂且面向治疗的基因回路。在开头部分列出了一个全面的开-关开关列表，这些开关分为转录、转录后、翻译和翻译后几类。随后将描述布尔逻辑门、合成哺乳动物振荡器和拨动开关。在医学应用部分将进一步综述几个合成基因网络，包括癌症治疗基因回路、免疫调节网络等。最后部分重点关注合成基因网络在药物发现、药物递送、受体激活基因回路和哺乳动物生物制造过程中的适用性。
