Healy C P, Deans T L
Department of Biomedical Engineering, University of Utah, Salt Lake City, UT 84112 USA.
J Biol Eng. 2019 May 3;13:39. doi: 10.1186/s13036-019-0170-7. eCollection 2019.
Persistent and complex problems arising with respect to human physiology and pathology have led to intense investigation into therapies and tools that permit more targeted outcomes and biomimetic responses to pathological conditions. A primary goal in mammalian synthetic biology is to build genetic circuits that exert fine control over cell behavior for next-generation biomedical applications. In pursuit of this, synthetic biologists have engineered cells endowed with genetic circuits with sensor that are capable of reacting to a variety of stimuli and responding with targeted behavior. Here, we highlight how synthetic biology approaches are being used to program cells with novel functions for therapeutic applications, and how they can be used in stem cells to improve differentiation outcomes. These approaches open the possibilities for engineering synthetic tissues for employing personalized medicine and to develop next-generation biomedical therapies.
人类生理学和病理学中出现的持续且复杂的问题，促使人们对能够实现更具针对性的结果以及对病理状况产生仿生反应的治疗方法和工具展开深入研究。哺乳动物合成生物学的一个主要目标是构建遗传回路，以便对细胞行为进行精确控制，用于下一代生物医学应用。为此，合成生物学家设计出了具有遗传回路的细胞，这些细胞配备了能够对各种刺激做出反应并产生靶向行为的传感器。在此，我们重点介绍合成生物学方法如何被用于为治疗应用对细胞进行编程，以及如何将其用于干细胞以改善分化结果。这些方法为设计用于个性化医疗的合成组织以及开发下一代生物医学疗法开辟了可能性。
