Pseudaminobacter granuli sp. nov., isolated from granules used in a wastewater treatment plant.

A Gram negative, aerobic, non-motile and rod-shaped bacterial strain designated as Gr-2 was isolated from granules used in a wastewater treatment plant in Korea, and its taxonomic position was investigated using a polyphasic approach. Strain Gr-2 grew at 18-37°C (optimum temperature, 30°C) and a pH of 6.0-8.0 (optimum pH, 7.0) on R2A agar medium. Based on 16S rRNA gene phylogeny, the novel strain showed a new branch within the genus Pseudaminobacter of the family Phyllobacteriaceae, and formed clusters with Pseudaminobacter defluvii THI 051 (98.9%) and Pseudaminobacter salicylatoxidans BN12 (98.7%). The G+C content of the genomic DNA was 63.6%. The predominant respiratory quinone was ubiquinone-10 (Q-10) and the major fatty acids were cyclo-C ω8c, C ω7c, and iso-C. The overall polar lipid patterns of Gr-2 were similar to those determined for the other Pseudaminobacter species. DNA-DNA relatedness values between strain Gr-2 and its closest phylogenetically neighbors were below 18%. Strain Gr-2 could be differentiated genotypically and phenotypically from the recognized species of the genus Pseudaminobacter. The isolate therefore represents a novel species, for which the name Pseudaminobacter granuli sp. nov. is proposed with the type strain Gr-2 (=KACC 18877 =LMG 29567).