用于测定海洋细菌革兰氏反应的非染色（氢氧化钾）法。
Nonstaining (KOH) method for determination of gram reactions of marine bacteria.
作者信息
Buck J D
出版信息
Appl Environ Microbiol. 1982 Oct;44(4):992-3. doi: 10.1128/aem.44.4.992-993.1982.
Abstract
A rapid nonstaining (KOH) method for the determination of the Gram reactions of bacteria is described, and its application to marine isolates is discussed. All gram-positive and gram-negative results obtained by Gram staining were confirmed by the KOH method. Gram-variable bacteria produced equivocal results.
摘要
本文描述了一种用于确定细菌革兰氏反应的快速非染色（氢氧化钾）方法，并讨论了其在海洋分离株中的应用。通过革兰氏染色获得的所有革兰氏阳性和革兰氏阴性结果均经氢氧化钾方法证实。革兰氏可变菌产生了不确定的结果。
相似文献
1
Nonstaining (KOH) method for determination of gram reactions of marine bacteria.用于测定海洋细菌革兰氏反应的非染色（氢氧化钾）法。
Appl Environ Microbiol. 1982 Oct;44(4):992-3. doi: 10.1128/aem.44.4.992-993.1982.
2
Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts.柳氏非染色氢氧化钾技术用于快速确定食源性和水源性细菌及酵母菌革兰氏反应的效果。
Appl Environ Microbiol. 1995 Oct;61(10):3756-8. doi: 10.1128/aem.61.10.3756-3758.1995.
3
Accuracy of the KOH and vancomycin tests in determining the Gram reaction of non-enterobacterial rods.氢氧化钾（KOH）和万古霉素试验在确定非肠杆菌属杆菌革兰氏反应中的准确性。
J Clin Microbiol. 1983 Oct;18(4):983-5. doi: 10.1128/jcm.18.4.983-985.1983.
4
Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria.有助于区分革兰氏阳性和革兰氏阴性厌氧细菌的快速方法。
J Clin Microbiol. 1981 Mar;13(3):444-8. doi: 10.1128/jcm.13.3.444-448.1981.
5
The KOH method for detecting the gram-staining potential of bacteria without staining.用于在不进行染色的情况下检测细菌革兰氏染色潜力的氢氧化钾方法。
Proc Annu Meet U S Anim Health Assoc. 1967;71:473-9.
6
7
[Supplementary methods for the determination of the Gram reaction of anaerobic pathogens].[厌氧病原体革兰氏反应测定的补充方法]
J Basic Microbiol. 1987;27(4):197-201. doi: 10.1002/jobm.3620270405.
8
Methods for distinguishing gram-positive from gram-negative bacteria.区分革兰氏阳性菌和革兰氏阴性菌的方法。
J Clin Microbiol. 1982 Dec;16(6):1157-9. doi: 10.1128/jcm.16.6.1157-1159.1982.
9
Evaluation of the KOH test and the antibiotic disk test in routine clinical anaerobic bacteriology.常规临床厌氧菌学中氢氧化钾试验和抗生素纸片试验的评估
J Clin Microbiol. 1988 Oct;26(10):2144-6. doi: 10.1128/jcm.26.10.2144-2146.1988.
10
Direct isolation of Yersinia pseudotuberculosis from fresh water in Japan.从日本淡水中直接分离出假结核耶尔森菌。
Appl Environ Microbiol. 1992 Aug;58(8):2688-90. doi: 10.1128/aem.58.8.2688-2690.1992.
引用本文的文献
1
Biodiversity and culture of prokaryotes inhabiting haloalkaline and meromictic Soap Lake, Washington, USA.美国华盛顿州具有盐碱化和半混合状态的肥皂湖中的原核生物的生物多样性与培养
Front Microbiol. 2025 Aug 6;16:1620605. doi: 10.3389/fmicb.2025.1620605. eCollection 2025.
2
sp. nov., isolated from a marine limpet .新种，从一种海洋帽贝中分离得到。
Int J Syst Evol Microbiol. 2025 Jul;75(7). doi: 10.1099/ijsem.0.006834.
3
Vibrio iocasae sp. nov., a bacterium isolated from the gill homogenate of bivalve lucinids Indoaustriella scarlatoi in intertidal mangrove sediments.新种湛江弧菌，一种从潮间带红树林沉积物中双壳类Lucinidae科的Indoaustriella scarlatoi鳃匀浆中分离出的细菌。
Antonie Van Leeuwenhoek. 2025 Jun 25;118(8):96. doi: 10.1007/s10482-025-02110-y.
4
Trujillonella humicola sp. nov., a siderophore-synthesizing bacterium isolated from black soil in Northeast China.土栖新鞘氨醇菌，一种从中国东北黑土中分离出的能合成铁载体的细菌。
Antonie Van Leeuwenhoek. 2025 Apr 22;118(5):73. doi: 10.1007/s10482-025-02081-0.
5
Three Novel Bacterial Species, Roseateles flavus sp. nov., Roseateles paludis sp. nov., and Roseateles hydrophilus sp. nov., Isolated from Various Water-Related Environments.从各种与水相关的环境中分离出的三个新细菌物种，即黄玫瑰色杆菌新种、沼泽玫瑰色杆菌新种和亲水玫瑰色杆菌新种。
Curr Microbiol. 2025 Apr 2;82(5):226. doi: 10.1007/s00284-025-04209-x.
6
sp. nov., a Non-Phytopathogenic Bacterium Isolated from the Seedlings of Spring Wheat ( L.).新种，一种从春小麦（L.）幼苗中分离出的非植物病原细菌。
Microorganisms. 2025 Feb 20;13(3):474. doi: 10.3390/microorganisms13030474.
7
Meridianimarinicoccus marinus sp. nov., Isolated from Tidal Flat.滨海子午线球菌新种，从潮滩分离得到。
Curr Microbiol. 2025 Mar 17;82(5):197. doi: 10.1007/s00284-025-04159-4.
8
Tabrizicola caldifontis sp. nov., Isolated from Hot Spring Sediment Sample.源自温泉沉积物样本中分离出的新物种——卡尔迪温泉塔布里齐菌。
Curr Microbiol. 2025 Mar 6;82(4):172. doi: 10.1007/s00284-025-04156-7.
9
Sphingosinicella rhizophila sp. nov., Isolated from Oat (Avena sativa L.) Rhizosphere Soil.嗜根鞘氨醇单胞菌新种，从燕麦（ Avena sativa L.）根际土壤中分离得到 。
Curr Microbiol. 2025 Mar 1;82(4):163. doi: 10.1007/s00284-025-04124-1.
10
Genomic and phenotypic characterisation of Leeuwenhoekiella obamensis sp. nov., a novel marine bacterium isolated from the surface water of a Japanese fishing port.从日本渔港表层水分离出的新型海洋细菌——奥巴马列文虎克氏菌的基因组和表型特征
Antonie Van Leeuwenhoek. 2025 Jan 27;118(2):49. doi: 10.1007/s10482-025-02061-4.
Zotero 插件