Shen Chwan-Li, Brackee Gordon, Song Xiao, Tomison Michael D, Finckbone VelvetLee, Mitchell Kelly T, Tang Lili, Chyu Ming-Chien, Dunn Dale M, Wang Jia-Sheng
Dept. of Pathology, Texas Tech Univ. Health Sciences Center, Lubbock, Tex., U.S.A.
Laboratory Animal Resources Center, Texas Tech Univ. Health Sciences Center, Lubbock, Tex., U.S.A.
J Food Sci. 2017 Sep;82(9):2192-2205. doi: 10.1111/1750-3841.13745. Epub 2017 Jul 28.
This work evaluates chronic safety in middle-aged ovariectomized rats supplemented with different dosages of green tea polyphenols (GTP) in drinking water. The experiment used 6-mo-old sham (n = 39) and ovariectomized (OVX, n = 143) female rats. All sham (n = 39) and 39 of the OVX animals received no GTP treatment and their samples were collected for outcome measures at baseline, 3 mo, and 6 mo (n = 13 per group for each). The remaining OVX animals were randomized into 4 groups receiving 0.15%, 0.5%, 1%, and 1.5% (n = 26 for each) of GTP (wt/vol), respectively, in drinking water for 3 and 6 mo. No mortality or abnormal treatment-related findings in clinical observations or ophthalmologic examinations were noted. No treatment-related macroscopic or microscopic findings were noted for animals administered 1.5% GTP supplementation. Throughout the study, there was no difference in the body weight among all OVX groups. In all OVX groups, feed intake and water consumption significantly decreased with GTP dose throughout the study period. At 6 mo, GTP intake did not affect hematology, clinical chemistry, and urinalysis, except for phosphorus and blood urea nitrogen (increased), total cholesterol, lactate dehydrogenase, and urine pH (decreased). This study reveals that the no-observed-adverse-effect level (NOAEL) of GTP is 1.5% (wt/vol) in drinking water, the highest dose used in this study.
本研究评估了在饮用水中补充不同剂量绿茶多酚（GTP）的中年去卵巢大鼠的慢性安全性。实验使用了6月龄的假手术组（n = 39）和去卵巢组（OVX，n = 143）雌性大鼠。所有假手术组（n = 39）和39只去卵巢动物未接受GTP处理，在基线、3个月和6个月时采集它们的样本用于结果测量（每组各13只）。其余去卵巢动物随机分为4组，分别在饮用水中接受0.15%、0.5%、1%和1.5%（每组n = 26）的GTP（重量/体积），持续3个月和6个月。在临床观察或眼科检查中未发现死亡或与处理相关的异常结果。给予1.5% GTP补充剂的动物未发现与处理相关的大体或微观结果。在整个研究过程中，所有去卵巢组的体重没有差异。在所有去卵巢组中，在整个研究期间，饲料摄入量和饮水量随GTP剂量显著降低。在6个月时，GTP摄入量除了对磷和血尿素氮（升高）、总胆固醇、乳酸脱氢酶和尿液pH（降低）有影响外，未影响血液学、临床化学和尿液分析。本研究表明，在本研究中使用的最高剂量饮用水中GTP未观察到有害作用水平（NOAEL）为1.5%（重量/体积）。
