Dept. of Food Science, Purdue University, 745 Agriculture Mall Dr, W Lafayette, IN, 47907, USA.
Dept. of Comparative Pathobiology, Purdue University, 625 Harrison St, W Lafayette, IN, 47907, USA.
Food Chem Toxicol. 2020 May;139:111254. doi: 10.1016/j.fct.2020.111254. Epub 2020 Mar 9.
Regular consumption of polyphenol-rich fruits and vegetables is associated with beneficial health outcomes. To increase polyphenol intakes, consumers are increasingly using herbal and botanical dietary supplements containing concentrated polyphenol extracts. However, the safety of this consumption modality has not been vetted. To address this, ovariectomized Sprague-Dawley (OVX-SD) rats were orally gavaged with purified blueberry polyphenols at 0-1000 mg total polyphenols/kg bw/d for 90d. No differences in behavior, body weight, or food consumption were observed. No tumors or macroscopic changes were observed, and histopathological analyses showed no differences among groups. Although several statistically significant differences between treatment and control groups were observed in urine (color and pH) and blood (monocyte count, total cholesterol, and chloride ion concentration) analyses, these parameters were within normal ranges and not considered biologically significant. Intestinal permeability assessed via FITC-dextran showed increased intestinal permeability in the highest dose, though no morphological differences were found throughout the gastrointestinal tract. Given the lack of other systemic changes, this finding is likely of minimal physiological importance. These results indicate a NOAEL for blueberry polyphenols in OVX-SD rats is ≥ 1000 mg total polyphenols/kg bw/d, which translates to a 70 kg human consuming ~10 g polyphenols. Keywords: Blueberry, Polyphenol, Sub-chronic toxicity.
经常食用富含多酚的水果和蔬菜与有益的健康结果有关。为了增加多酚的摄入量，消费者越来越多地使用含有浓缩多酚提取物的草药和植物性膳食补充剂。然而，这种消费方式的安全性尚未经过审查。为了解决这个问题，对去卵巢 Sprague-Dawley（OVX-SD）大鼠进行了 90 天的口服蓝莓多酚纯化物处理，剂量为 0-1000mg 总多酚/kg bw/d。未观察到行为、体重或食物消耗的差异。未观察到肿瘤或宏观变化，组织病理学分析显示各组之间无差异。尽管尿液（颜色和 pH 值）和血液（单核细胞计数、总胆固醇和氯离子浓度）分析中观察到治疗组和对照组之间存在几个统计学上显著的差异，但这些参数在正常范围内，不被认为具有生物学意义。通过 FITC-葡聚糖评估的肠道通透性显示最高剂量时肠道通透性增加，但整个胃肠道未发现形态差异。鉴于没有其他全身变化，这一发现可能具有最小的生理重要性。这些结果表明，蓝莓多酚在 OVX-SD 大鼠中的无观察到不良作用水平（NOAEL）为 ≥1000mg 总多酚/kg bw/d，这相当于 70kg 人摄入约 10g 多酚。关键词：蓝莓、多酚、亚慢性毒性。
