YRNA 表达谱在肾透明细胞癌中发生改变。

YRNA Expression Profiles are Altered in Clear Cell Renal Cell Carcinoma.

机构信息

Department of Urology, University Hospital Bonn, Bonn, Germany.

Institute of Pathology, University Hospital Bonn, Bonn, Germany.

出版信息

Eur Urol Focus. 2018 Mar;4(2):260-266. doi: 10.1016/j.euf.2016.08.004. Epub 2016 Aug 23.

DOI:10.1016/j.euf.2016.08.004

PMID:28753785

Abstract

BACKGROUND

Noncoding RNAs play an important role in human carcinogenesis. YRNAs, a novel class of noncoding RNAs, have been identified as biomarkers in breast cancer patients.

OBJECTIVE

To test the hypothesis that YRNA expression is dysregulated in clear cell renal cell carcinoma (ccRCC).

DESIGN, SETTING, AND PARTICIPANTS: We first measured the expression of all known YRNAs (hY1, hY3, hY4, and hY5) in a screening cohort (30 ccRCC and 15 normal renal tissues). Subsequently, hY3 and hY4 were validated in an independent cohort (88 ccRCC and 59 normal renal tissues). Finally, hY3 and hY4 levels in serum samples from 30 ccRCC and 15 control individuals were measured. YRNAs were detected using quantitative real-time polymerase chain reaction.

OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS

Relative expression values were analyzed using the Mann-Whitney-U test and Kaplan Meier estimates.

RESULTS AND LIMITATIONS

Expression of hY3 and hY4 was increased in ccRCC samples compared with normal renal tissue, whereas hY1 and hY5 levels were similar. Expression levels of hY4 correlated with ccRCC stage and the presence of lymph node metastases. Neither hY3 nor hY4 were circulating at different levels in ccRCC patients and control individuals.

CONCLUSIONS

The expression of hY3 and hY4 is altered in ccRCC and associated with advanced disease.

PATIENT SUMMARY

In this report we studied the expression of noncoding YRNA in clear cell renal cell carcinoma tissue. We observed increased hY3 and hY4 expression levels in cancer tissues. However, expression levels were not different in the serum of patients with cancer or benign disease.

摘要

背景

非编码 RNA 在人类癌症发生中发挥着重要作用。YRNA 是一类新的非编码 RNA，已被鉴定为乳腺癌患者的生物标志物。

目的

检验 YRNA 表达在透明细胞肾细胞癌（ccRCC）中失调的假说。

设计、地点和参与者：我们首先在筛选队列（30 例 ccRCC 和 15 例正常肾组织）中测量了所有已知 YRNA（hY1、hY3、hY4 和 hY5）的表达。随后，在独立队列（88 例 ccRCC 和 59 例正常肾组织）中验证了 hY3 和 hY4。最后，测量了 30 例 ccRCC 和 15 例对照个体血清样本中的 hY3 和 hY4 水平。使用实时定量聚合酶链反应检测 YRNA。