Bland M M, Levings C S, Matzinger D F
Mol Gen Genet. 1986 Jul;204(1):8-16. doi: 10.1007/BF00330180.
A transcribed segment of mitochondrial DNA (mtDNA) from Nicotiana tabacum contains the F0-ATPase subunit 9 gene, an open reading frame with homology to the E. coli small subunit ribosomal protein S13 and an open reading frame with homology to a portion of the mammalian "URF 1" protein, recently shown to be a component of the NADH:ubiquinone reductase complex (NADH:Q 1). The transcriptional patterns of the tobacco ATPase 9 gene and S13-like open reading frame share eight RNA species indicating the two sequences are part of the same transcriptional unit. A maize mtDNA fragment contains the S13 homologous sequence and the NADH:Q 1 homologous sequence in an orientation similar to tobacco. The S13-like sequence is present as a single copy in maize and tobacco, as two copies in wheat, and is absent in pea and bean. We discuss the distribution and orientation of the S13-like and "URF 1"-like sequences and the possibility that they are active genes.
烟草线粒体DNA（mtDNA）的一个转录片段包含F0 - ATP酶亚基9基因、一个与大肠杆菌小亚基核糖体蛋白S13具有同源性的开放阅读框以及一个与哺乳动物“URF 1”蛋白的一部分具有同源性的开放阅读框，最近发现该蛋白是NADH：泛醌还原酶复合体（NADH：Q 1）的一个组成部分。烟草ATP酶9基因和S13样开放阅读框的转录模式共有8种RNA种类，表明这两个序列是同一转录单元的一部分。一个玉米mtDNA片段含有S13同源序列和NADH：Q 1同源序列，其方向与烟草相似。S13样序列在玉米和烟草中以单拷贝形式存在，在小麦中以两个拷贝形式存在，在豌豆和菜豆中不存在。我们讨论了S13样和“URF 1”样序列的分布和方向以及它们作为活性基因的可能性。
