Hu Yongbo, Ma Zhen, He Yiming, Liu Wei, Su Yu, Tang Zongbin
Department of General Surgery, Xiantao First People's Hospital Affiliated to Yangtze University, Xiantao, Hubei, China.
Department of General Surgery, Xiantao First People's Hospital Affiliated to Yangtze University, Xiantao, Hubei, China.
Biochem Biophys Res Commun. 2017 Sep 30;491(4):926-931. doi: 10.1016/j.bbrc.2017.07.137. Epub 2017 Jul 25.
The objective of this study was to determine the expression of long-chain non-coding RNA SNHG1 (lncRNA-SNHG1) in gastric carcinoma and explore its function on cancer cell proliferation.
The expression of lncRNA-SNHG1 in tumor tissues and corresponding adjacent tissues from 50 patients with gastric cancer was detected with realtime-PCR. The relationships between the expression of lncRNA-SNHG1 and clinicopathological features of gastric cancer patients were analyzed. Survival analysis was performed to study the correlation between lncRNA SNHG1 expression and patient prognosis. To assess the effect of LncRNA SNHG1 on proliferation in cancer cells, cell viability and colony formation assays were conducted when lncRNA SNHG1 was upregulated or downregulated by Lentivirus or plasmid in gastric cancer cells. Furthermore, in vivo tumor assay was performed to confirm the impact of lncRNA SNHG1 on proliferation of gastric cancer.
The expression of lncRNA SNHG1 in gastric cancer tissues was significantly higher than that in adjacent tissues and was correlated with TNM stage, T stage, and lymph node metastasis. The survival time of patients with higher expression level of lncRNA-SNHG1 was significantly lower than that of the lower expression level. LncRNA-SNHG1 accelerated the proliferation of gastric cancer cells obviously and increased the expression of DNMT1.
LncRNA SNHG1 promotes DNMT1 expression, which facilitates the gastric cancer proliferation.
本研究旨在测定长链非编码RNA SNHG1（lncRNA-SNHG1）在胃癌中的表达，并探讨其对癌细胞增殖的作用。
采用实时定量PCR检测50例胃癌患者肿瘤组织及相应癌旁组织中lncRNA-SNHG1的表达。分析lncRNA-SNHG1表达与胃癌患者临床病理特征之间的关系。进行生存分析以研究lncRNA SNHG1表达与患者预后的相关性。为评估LncRNA SNHG1对癌细胞增殖的影响，当通过慢病毒或质粒在胃癌细胞中上调或下调lncRNA SNHG1时，进行细胞活力和集落形成试验。此外，进行体内肿瘤试验以证实lncRNA SNHG1对胃癌增殖的影响。
lncRNA SNHG1在胃癌组织中的表达明显高于癌旁组织，且与TNM分期、T分期及淋巴结转移相关。lncRNA-SNHG1表达水平较高的患者生存时间明显低于表达水平较低的患者。LncRNA-SNHG1明显促进胃癌细胞增殖并增加DNMT1的表达。
LncRNA SNHG1促进DNMT1表达，从而促进胃癌增殖。
