• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码RNA Snhg1作为miR-338的不可降解海绵，可促进原发性食管癌细胞中原癌基因CST3的表达。

LncRNA Snhg1, a non-degradable sponge for miR-338, promotes expression of proto-oncogene CST3 in primary esophageal cancer cells.

作者信息

Yan Yan, Fan Qingxia, Wang Liping, Zhou Yue, Li Jianhua, Zhou Kun

机构信息

Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Department of Medical Imaging, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

出版信息

Oncotarget. 2017 May 30;8(22):35750-35760. doi: 10.18632/oncotarget.16189.

DOI:10.18632/oncotarget.16189
PMID:28423738
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5482614/
Abstract

Competing endogenous RNA (ceRNA) is a newly proposed mechanism that describes a crosstalk among lncRNAs, mRNAs and their shared miRNAs. In this study, the role of miR-338-3p (miR-338) in the progression of esophageal cancer and its involve in the ceRNA regulatory circuit lncRNA-Snhg1/CST3 were explored. MiR-338 displayed a 30% decreased expression in esophageal squamous cell carcinoma tissues compared with the adjacent. Then, proto-oncogene CST3 was predicted and validated as a target gene of miR-338. Gain-and-loss-function experiments indicated that miR-338 suppressed expression of CST3 protein (also Cystatin C, CysC), promoted expression of apoptotic proteins caspase-8/3, attenuated esophageal carcinoma cell growth and induced its apoptosis. In addition, lncRNA-Snhg1 was significantly upregulated in esophageal carcinoma tissues and promoted esophageal carcinoma cell growth. Furthermore, our results from bioinformatics, luciferase reporter gene and RNA pull-down assays indicated that Snhg1 could be directly bound by miR-338. Snhg1 acted as a non-degradable sponge to relieve the suppression on CST3 caused by miR-338. In conclusion, lncRNA-Snhg1 promoted cell proliferation by acting as a non-degradable sponge for the tumor suppressor miR-338 in esophageal cancer cells.

摘要

竞争性内源RNA（ceRNA）是一种新提出的机制，描述了长链非编码RNA（lncRNA）、信使核糖核酸（mRNA）及其共享的微小核糖核酸（miRNA）之间的相互作用。在本研究中，探讨了miR-338-3p（miR-338）在食管癌进展中的作用及其在ceRNA调控回路lncRNA-Snhg1/CST3中的参与情况。与癌旁组织相比，miR-338在食管鳞状细胞癌组织中的表达降低了30%。随后，原癌基因CST3被预测并验证为miR-338的靶基因。功能获得和缺失实验表明，miR-338抑制CST3蛋白（即胱抑素C，CysC）的表达，促进凋亡蛋白caspase-8/3的表达，减弱食管癌细胞的生长并诱导其凋亡。此外，lncRNA-Snhg1在食管癌组织中显著上调，并促进食管癌细胞的生长。此外，我们通过生物信息学、荧光素酶报告基因和RNA下拉实验得到的结果表明，Snhg1可被miR-338直接结合。Snhg1作为一种不可降解的海绵，可减轻miR-338对CST3的抑制作用。总之，lncRNA-Snhg1在食管癌细胞中作为肿瘤抑制性miR-338的不可降解海绵，促进细胞增殖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/62f89d1f316e/oncotarget-08-35750-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/473070fc53db/oncotarget-08-35750-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/ddfca4f49b3b/oncotarget-08-35750-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/657624f5c0b3/oncotarget-08-35750-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/99fa15e080b9/oncotarget-08-35750-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/700604e63136/oncotarget-08-35750-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/62f89d1f316e/oncotarget-08-35750-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/473070fc53db/oncotarget-08-35750-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/ddfca4f49b3b/oncotarget-08-35750-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/657624f5c0b3/oncotarget-08-35750-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/99fa15e080b9/oncotarget-08-35750-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/700604e63136/oncotarget-08-35750-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c432/5482614/62f89d1f316e/oncotarget-08-35750-g006.jpg

相似文献

1
LncRNA Snhg1, a non-degradable sponge for miR-338, promotes expression of proto-oncogene CST3 in primary esophageal cancer cells.长链非编码RNA Snhg1作为miR-338的不可降解海绵，可促进原发性食管癌细胞中原癌基因CST3的表达。
Oncotarget. 2017 May 30;8(22):35750-35760. doi: 10.18632/oncotarget.16189.
2
Downregulation of SNHG1 suppresses cell proliferation and invasion by regulating Notch signaling pathway in esophageal squamous cell cancer.SNHG1 的下调通过调节 Notch 信号通路抑制食管鳞状细胞癌中的细胞增殖和侵袭。
Cancer Biomark. 2017 Dec 12;21(1):89-96. doi: 10.3233/CBM-170286.
3
The long noncoding RNA SNHG1 regulates colorectal cancer cell growth through interactions with EZH2 and miR-154-5p.长链非编码 RNA SNHG1 通过与 EZH2 和 miR-154-5p 的相互作用调节结直肠癌细胞生长。
Mol Cancer. 2018 Sep 28;17(1):141. doi: 10.1186/s12943-018-0894-x.
4
Upregulated lncRNA SNHG1 contributes to progression of non-small cell lung cancer through inhibition of miR-101-3p and activation of Wnt/β-catenin signaling pathway.上调的长链非编码RNA SNHG1通过抑制miR-101-3p和激活Wnt/β-连环蛋白信号通路促进非小细胞肺癌进展。
Oncotarget. 2017 Mar 14;8(11):17785-17794. doi: 10.18632/oncotarget.14854.
5
LncRNA-TUSC7/miR-224 affected chemotherapy resistance of esophageal squamous cell carcinoma by competitively regulating DESC1.长链非编码 RNA-TUSC7/miR-224 通过竞争性调节 DESC1 影响食管鳞癌细胞的化疗耐药性。
J Exp Clin Cancer Res. 2018 Mar 12;37(1):56. doi: 10.1186/s13046-018-0724-4.
6
Long non-coding RNA SNHG1 functions as a competitive endogenous RNA to regulate PDCD4 expression by sponging miR-195-5p in hepatocellular carcinoma.长链非编码 RNA SNHG1 通过海绵吸附 miR-195-5p 来调节肝细胞癌中 PDCD4 的表达，作为竞争性内源性 RNA 发挥作用。
Gene. 2019 Sep 25;714:143994. doi: 10.1016/j.gene.2019.143994. Epub 2019 Jul 19.
7
Long noncoding RNA SNHG1 promotes non-small cell lung cancer progression by up-regulating MTDH via sponging miR-145-5p.长链非编码 RNA SNHG1 通过海绵吸附 miR-145-5p 而上调 MTDH 促进非小细胞肺癌进展。
FASEB J. 2018 Jul;32(7):3957-3967. doi: 10.1096/fj.201701237RR. Epub 2018 Feb 21.
8
lncRNA SNHG1 negatively regulates miRNA‑101‑3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma.长链非编码 RNA SNHG1 通过负调控 miRNA-101-3p 来增强 ROCK1 的表达，从而促进骨肉瘤细胞的增殖、迁移和侵袭。
Int J Mol Med. 2019 Mar;43(3):1157-1166. doi: 10.3892/ijmm.2018.4039. Epub 2018 Dec 20.
9
Knockdown of lncRNA SNHG1 attenuated Aβ-inudced neuronal injury via regulating KREMEN1 by acting as a ceRNA of miR-137 in neuronal cells.敲低 lncRNA SNHG1 通过作为 miR-137 的 ceRNA 调节 KREMEN1 减轻神经元细胞中 Aβ诱导的神经元损伤。
Biochem Biophys Res Commun. 2019 Oct 20;518(3):438-444. doi: 10.1016/j.bbrc.2019.08.033. Epub 2019 Aug 23.
10
Long non-coding RNA HOTAIR functions as miRNA sponge to promote the epithelial to mesenchymal transition in esophageal cancer.长链非编码 RNA HOTAIR 通过作为 miRNA 海绵促进食管癌中的上皮间质转化。
Biomed Pharmacother. 2017 Jun;90:888-896. doi: 10.1016/j.biopha.2017.03.103. Epub 2017 Apr 22.

引用本文的文献

1
Noncoding RNAs as regulators of FOSL1 in cancer.非编码RNA作为癌症中FOSL1的调节因子
Front Immunol. 2025 Aug 1;16:1599674. doi: 10.3389/fimmu.2025.1599674. eCollection 2025.
2
Roles of lncRNA in the crosstalk between osteogenesis and angiogenesis in the bone microenvironment.长链非编码RNA在骨微环境中骨生成与血管生成相互作用中的作用
J Zhejiang Univ Sci B. 2025 Feb 25;26(2):107-123. doi: 10.1631/jzus.B2300607.
3
Osteosarcoma in a ceRNET perspective.中枢神经系统外尤文肉瘤的临床特征。

本文引用的文献

1
Long noncoding RNA SNHG1 predicts a poor prognosis and promotes hepatocellular carcinoma tumorigenesis.长链非编码 RNA SNHG1 预测预后不良，并促进肝癌肿瘤发生。
Biomed Pharmacother. 2016 May;80:73-79. doi: 10.1016/j.biopha.2016.02.036. Epub 2016 Mar 15.
2
Cytoplasmic long noncoding RNAs are frequently bound to and degraded at ribosomes in human cells.细胞质长链非编码RNA在人类细胞中经常与核糖体结合并在核糖体处降解。
RNA. 2016 Jun;22(6):867-82. doi: 10.1261/rna.053561.115. Epub 2016 Apr 18.
3
Expression of cystatin C and its effect on EC9706 cells in esophageal carcinoma.
J Biomed Sci. 2024 Jun 5;31(1):59. doi: 10.1186/s12929-024-01049-y.
4
LncRNA SNHG1 acts as a ceRNA for miR-216a-3p to regulate TMBIM6 expression in esophageal squamous cell carcinoma.长链非编码RNA SNHG1作为miR-216a-3p的竞争性内源RNA，在食管鳞状细胞癌中调节TMBIM6的表达。
J Cancer. 2024 Apr 8;15(10):3128-3139. doi: 10.7150/jca.95127. eCollection 2024.
5
Type 2 Cystatins and Their Roles in the Regulation of Human Immune Response and Cancer Progression.2型胱抑素及其在人类免疫反应调节和癌症进展中的作用。
Cancers (Basel). 2023 Nov 10;15(22):5363. doi: 10.3390/cancers15225363.
6
LncRNA SNHG1: role in tumorigenesis of multiple human cancers.长链非编码RNA SNHG1：在多种人类癌症肿瘤发生中的作用
Cancer Cell Int. 2023 Sep 8;23(1):198. doi: 10.1186/s12935-023-03018-1.
7
lncRNA TPT1-AS1 promotes cell migration and invasion in esophageal squamous-cell carcinomas by regulating the miR-26a/HMGA1 axis.长链非编码RNA TPT1-AS1通过调控miR-26a/HMGA1轴促进食管鳞状细胞癌的细胞迁移和侵袭。
Open Med (Wars). 2023 Feb 14;18(1):20220533. doi: 10.1515/med-2022-0533. eCollection 2023.
8
M6A-related lncRNAs predict clinical outcome and regulate the tumor immune microenvironment in hepatocellular carcinoma.M6A 相关长链非编码 RNA 预测肝细胞癌的临床结局并调节肿瘤免疫微环境。
BMC Cancer. 2022 Aug 9;22(1):867. doi: 10.1186/s12885-022-09925-2.
9
Integrative Analysis and Experimental Validation Indicated That SNHG17 Is a Prognostic Marker in Prostate Cancer and a Modulator of the Tumor Microenvironment via a Competitive Endogenous RNA Regulatory Network.整合分析和实验验证表明，SNHG17 是前列腺癌的预后标志物，并通过竞争性内源性 RNA 调控网络调节肿瘤微环境。
Oxid Med Cell Longev. 2022 Jul 12;2022:1747604. doi: 10.1155/2022/1747604. eCollection 2022.
10
Long non-coding RNA AC087388.1 as a novel biomarker in colorectal cancer.长链非编码 RNA AC087388.1 作为结直肠癌的新型生物标志物。
BMC Cancer. 2022 Feb 21;22(1):196. doi: 10.1186/s12885-022-09282-0.
胱抑素C在食管癌中的表达及其对EC9706细胞的影响。
Int J Clin Exp Pathol. 2015 Sep 1;8(9):10102-11. eCollection 2015.
4
Non-Coding RNAs in Primary Liver Cancer.原发性肝癌中的非编码 RNA。
Front Med (Lausanne). 2015 Jun 4;2:36. doi: 10.3389/fmed.2015.00036. eCollection 2015.
5
MiR-338-3p inhibits epithelial-mesenchymal transition in gastric cancer cells by targeting ZEB2 and MACC1/Met/Akt signaling.微小RNA-338-3p通过靶向锌指E盒结合蛋白2以及甲硫氨酸腺苷基转移酶1A/间充质上皮转化因子/蛋白激酶B信号通路抑制胃癌细胞的上皮-间质转化
Oncotarget. 2015 Jun 20;6(17):15222-34. doi: 10.18632/oncotarget.3835.
6
Noncoding RNA small nucleolar RNA host gene 1 promote cell proliferation in nonsmall cell lung cancer.非编码RNA小核仁RNA宿主基因1促进非小细胞肺癌细胞增殖。
Indian J Cancer. 2014 Mar;51 Suppl 3:e99-e102. doi: 10.4103/0019-509X.154092.
7
RNA-RNA interactions in gene regulation: the coding and noncoding players.RNA 与 RNA 相互作用在基因调控中的作用：编码与非编码的调控者。
Trends Biochem Sci. 2015 May;40(5):248-56. doi: 10.1016/j.tibs.2015.03.001. Epub 2015 Mar 25.
8
Involvement of cysteine proteases in cancer.半胱氨酸蛋白酶与癌症的关系。
Curr Med Chem. 2015;22(8):944-57. doi: 10.2174/0929867321666141106115624.
9
MicroRNAs: biogenesis, roles for carcinogenesis and as potential biomarkers for cancer diagnosis and prognosis.微小RNA：生物合成、在致癌过程中的作用以及作为癌症诊断和预后的潜在生物标志物
Asian Pac J Cancer Prev. 2014;15(18):7489-97. doi: 10.7314/apjcp.2014.15.18.7489.
10
Competing endogenous RNA: the key to posttranscriptional regulation.竞争性内源RNA：转录后调控的关键
ScientificWorldJournal. 2014 Feb 2;2014:896206. doi: 10.1155/2014/896206. eCollection 2014.