Alwael Hussain A, Naik Poornananda M, Al-Khayri Jameel M
Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Hassa, Saudi Arabia.
Methods Mol Biol. 2017;1637:215-226. doi: 10.1007/978-1-4939-7156-5_18.
Somatic embryogenesis is considered the most effective method for commercial propagation of date palm. However, the limitation of obtaining synchronized development of somatic embryos remains an impediment. The synchronization of somatic embryo development is ideal for the applications to produce artificial seeds. Abscisic acid (ABA) is associated with stress response and influences in vitro growth and development. This chapter describes an effective method to achieve synchronized development of somatic embryos in date palm cell suspension culture. Among the ABA concentrations tested (0, 1, 10, 50, 100 μM), the best synchronized growth was obtained in response to 50-100 μM. Here we provide a comprehensive protocol for in vitro plant regeneration of date palm starting with shoot-tip explant, callus initiation and growth, cell suspension establishment, embryogenesis synchronization with ABA treatment, somatic embryo germination, and rooting as well as acclimatized plantlet establishment.
体细胞胚胎发生被认为是枣椰树商业繁殖最有效的方法。然而，获得体细胞胚胎同步发育的局限性仍然是一个障碍。体细胞胚胎发育的同步化对于生产人工种子的应用来说是理想的。脱落酸（ABA）与应激反应相关，并影响体外生长和发育。本章描述了一种在枣椰树细胞悬浮培养中实现体细胞胚胎同步发育的有效方法。在所测试的ABA浓度（0、1、10、50、100μM）中，50 - 100μM的浓度下获得了最佳的同步生长。在这里，我们提供了一个从茎尖外植体开始，经过愈伤组织诱导和生长、细胞悬浮系建立、ABA处理使胚胎发生同步化、体细胞胚胎萌发、生根以及驯化植株建立的枣椰树体外植株再生的综合方案。
