Spínola Vítor, Castilho Paula C
CQM - Centro de Química da Madeira, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, Portugal.
CQM - Centro de Química da Madeira, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, Portugal.
Phytochemistry. 2017 Nov;143:29-35. doi: 10.1016/j.phytochem.2017.07.006. Epub 2017 Jul 26.
The study was performed to assess, for the first time, the in vitro anti-diabetic potential of ten Asteraceae plant extracts to inhibit the activity of digestive enzymes (α-amylase, α-, β-glucosidases and lipase) responsible for hydrolysis/digestion of sugar and lipids. Prevention of advanced glycation end-products (AGEs) formation was evaluated in bovine serum albumin/ribose glycation reaction model. The phytochemical profiles and caffeoylquinic acids (CQAs) contents were determined for the methanolic extract of each plant. Analyzed plant extracts exhibited significant inhibitory activity against key digestive enzymes linked to type II diabetes and obesity. A strong inhibition was observed for glucosidases and mild activity towards amylase and lipase (compared to reference compounds). Moreover, some extracts exhibited potent ability to prevent formation of AGEs, implicated in some diabetic complications. Caffeoylquinic acids were dominant in all plant extracts and findings demonstrate that these compounds are the most relevant hypoglycemic and anti-glycation agents. From the obtained results, Argyranthemum pinnatifidum, Helichrysum melaleucum, and Phagnalon lowei are good candidates for further development of phyto-pharmaceutical preparations as complementary therapy for diabetes and obesity control.
本研究首次对十种菊科植物提取物的体外抗糖尿病潜力进行评估，以抑制负责糖和脂质水解/消化的消化酶（α-淀粉酶、α-、β-葡萄糖苷酶和脂肪酶）的活性。在牛血清白蛋白/核糖糖基化反应模型中评估了晚期糖基化终产物（AGEs）形成的预防情况。测定了每种植物甲醇提取物的植物化学特征和咖啡酰奎宁酸（CQAs）含量。分析的植物提取物对与II型糖尿病和肥胖相关的关键消化酶表现出显著的抑制活性。观察到对葡萄糖苷酶有强烈抑制作用，对淀粉酶和脂肪酶有轻微活性（与参考化合物相比）。此外，一些提取物表现出强大的预防AGEs形成的能力，AGEs与一些糖尿病并发症有关。咖啡酰奎宁酸在所有植物提取物中占主导地位，研究结果表明这些化合物是最相关的降血糖和抗糖基化剂。从获得的结果来看，裂叶蓬蒿、白叶蜡菊和洛氏绢蒿是进一步开发植物药物制剂作为糖尿病和肥胖控制辅助疗法的良好候选者。
