对第一代基因组编辑突变体进行表型分析：一种新标准？
Phenotyping first-generation genome editing mutants: a new standard?
作者信息
Teboul Lydia, Murray Stephen A, Nolan Patrick M
机构信息
The Mary Lyon Centre, MRC Harwell Institute, Harwell Science Campus, Didcot, Oxfordshire, UK.
The Jackson Laboratory, Bar Harbor, ME, USA.
出版信息
Mamm Genome. 2017 Aug;28(7-8):377-382. doi: 10.1007/s00335-017-9711-x. Epub 2017 Jul 29.
Abstract
The unprecedented efficiency of the CRISPR/Cas9 system in genome engineering has opened the prospect of employing mutant founders for phenotyping cohorts, thus accelerating research projects by circumventing the requirement to generate cohorts using conventional two- or three-generation crosses. However, these first-generation mutants are often genetic mosaics, with a complex and difficult to define genetic make-up. Here, we discuss the potential benefits, challenges and scientific validity of such models.
摘要
CRISPR/Cas9系统在基因组工程中前所未有的效率为利用突变体创建者进行表型队列研究开辟了前景，从而通过规避使用传统两代或三代杂交来创建队列的要求，加速了研究项目。然而，这些第一代突变体往往是基因嵌合体，其基因组成复杂且难以定义。在此，我们讨论此类模型的潜在益处、挑战及科学有效性。
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