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用于从斑马鱼幼体引出视网膜电图的新型白光发光二极管光刺激系统。

New photic stimulating system with white light-emitting diodes to elicit electroretinograms from zebrafish larvae.

作者信息

Matsubara Hisashi, Matsui Yoshitsugu, Miyata Ryohei, Nishimura Yuhei, Yamamoto Tetsuro, Tanaka Toshio, Kondo Mineo

机构信息

Department of Ophthalmology, Graduate School of Medicine, Mie University, 2-174 Edobashi, Tsu-city, Mie, 514-8507, Japan.

Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Graduate School of Medicine, Mie University, 2-174 Edobashi, Tsu-city, Mie, 514-8507, Japan.

出版信息

Doc Ophthalmol. 2017 Oct;135(2):147-154. doi: 10.1007/s10633-017-9602-1. Epub 2017 Jul 29.

DOI:10.1007/s10633-017-9602-1
PMID:28756596
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5606953/
Abstract

PURPOSE

The zebrafish is an established animal model commonly used in biological, neuroscience, and genetic research. We have developed a new light stimulating system using white light-emitting diodes (LEDs) to elicit ERGs from zebrafish larvae. The purpose of this study was to record full-field ERGs and to evaluate the inter-trial reliability of the ERGs recorded with our system from zebrafish larvae.

METHODS

The stimulating device used white LEDs that were attached to a stereomicroscope, and the location of the recording electrode on the cornea could be monitored while the eye was being stimulated. Full-field scotopic and photopic ERGs were recorded from larvae at the age of 5-7 days post-fertilization (dpf). Intensity-response curves were constructed from the ERGs. Inter-trial reliability of the ERGs recorded by our system was evaluated.

RESULTS

This stimulating system could be used for efficient and reliable ERG recordings from 5-7 dpf larvae. The amplitudes, implicit times, and the waveforms of the scotopic and photopic ERGs were similar to those reported in earlier studies. Inter-trial reliability of the amplitudes of the photopic ERG b-waves was excellent with an intra-class correlation coefficient of 0.98.

CONCLUSION

We conclude that this new light stimulation system using white LEDs attached to a stereomicroscope will be helpful in recording reliable ERGs from zebrafish larvae.

摘要

目的

斑马鱼是一种常用于生物学、神经科学和遗传学研究的成熟动物模型。我们开发了一种新的光刺激系统，该系统使用白色发光二极管（LED）从斑马鱼幼虫中引出视网膜电图（ERG）。本研究的目的是记录全视野ERG，并评估我们的系统记录的斑马鱼幼虫ERG的试验间可靠性。

方法

刺激装置使用附着在体视显微镜上的白色LED，在刺激眼睛时可以监测角膜上记录电极的位置。从受精后5-7天（dpf）的幼虫记录全视野暗视和明视ERG。根据ERG构建强度-反应曲线。评估我们的系统记录的ERG的试验间可靠性。

结果

该刺激系统可用于高效、可靠地记录5-7 dpf幼虫的ERG。暗视和明视ERG的振幅、隐含时间和波形与早期研究报道的相似。明视ERG b波振幅的试验间可靠性极佳，组内相关系数为0.98。

结论

我们得出结论，这种使用附着在体视显微镜上的白色LED的新型光刺激系统将有助于记录斑马鱼幼虫可靠的ERG。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/0b661e4f17b9/10633_2017_9602_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/f6ee61516b7d/10633_2017_9602_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/0c4cdcd67a5d/10633_2017_9602_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/90ca693d7700/10633_2017_9602_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/7684f09707d2/10633_2017_9602_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/0b661e4f17b9/10633_2017_9602_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/f6ee61516b7d/10633_2017_9602_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/0c4cdcd67a5d/10633_2017_9602_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/90ca693d7700/10633_2017_9602_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/7684f09707d2/10633_2017_9602_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d267/5606953/0b661e4f17b9/10633_2017_9602_Fig5_HTML.jpg

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