Department of Chemistry and ‡Department of Biotechnology, Indian Institute of Technology , Kharagpur 721302, WB, India.
J Phys Chem B. 2017 Aug 31;121(34):8162-8170. doi: 10.1021/acs.jpcb.7b06231. Epub 2017 Aug 17.
Ionic liquids (ILs) are considered as "green solvents" for more than 2 decades. However, recent studies suggest that some ILs exhibit greater toxicity compared to common solvents. As a proactive effort to better understand the molecular origin of the cytotoxicity, the work herein presents the systemic characterization of the interaction between model membrane composed of fatty acids and popular imidazolium-based hydrophilic IL. The fusion kinetics between the vesicles demonstrates the swelling of the vesicle. Further, membrane fluidity is determined using the isomerization kinetics of a lipophilic dye, merocyanine-540, and in the presence of IL, the fluidity of the inner water pool of the vesicle is increased. The results can be directly correlated to the cytotoxicity generated by IL in K562 cell, a human erythroleukemic cell line. High-concentration IL ruptures the cell membrane and causes membrane permeabilization. Thus, the results would help to facilitate the rational design of nontoxic ILs.
离子液体（ILs）作为“绿色溶剂”已有二十多年的历史。然而，最近的研究表明，与常见溶剂相比，一些 ILs 表现出更大的毒性。作为更好地理解细胞毒性分子起源的积极努力，本文系统地研究了由脂肪酸组成的模型膜与常用的咪唑基亲水性 IL 之间的相互作用。囊泡的融合动力学表明囊泡的肿胀。此外，使用亲脂性染料变色氰-540 的异构化动力学来确定膜流动性，并且在 IL 的存在下，囊泡的内水相的流动性增加。结果可以直接与 IL 在 K562 细胞（一种人类红白血病细胞系）中产生的细胞毒性相关联。高浓度的 IL 会破坏细胞膜并导致膜通透性增加。因此，这些结果将有助于促进无毒 IL 的合理设计。
