Department of Analytical Chemistry, Institute of Biomolecules (INBIO), Faculty of Sciences, CEI-MAR, University of Cadiz, Campus Rio San Pedro, ES-11510 Puerto Real, Cadiz, Spain.
Department of Integrated Sciences, Faculty of Experimental Sciences, CEI-MAR, University of Huelva, Avda. Fuerzas Armadas, ES-21071 Huelva, Spain.
Mar Pollut Bull. 2017 Nov 15;124(1):336-348. doi: 10.1016/j.marpolbul.2017.07.039. Epub 2017 Jul 27.
A comprehensive approach to chemical accumulation and biological effects of short-term Cu exposure in juveniles of European seabass (Dicentrarchus labrax) has been achieved. Fish were exposed to 0.01-10mgL nominal Cu concentrations for 24-96h. Metal concentrations in water and gills, liver, muscle and brain tissues were studied along with oxidative stress biomarkers (superoxide dismutase, catalase, glutathione peroxidase, lipid peroxidation). Induction of oxidative damage was observed in all the organs with differential antioxidant responses; gills appearing as the most sensitive from low environmentally water Cu concentrations as 0.01mgL. Histopathological alterations were also observed in liver and gills, even without a significant Cu accumulation. The results show that the combination of oxidative stress parameters, particularly lipid peroxidation and glutathione peroxidase activities, and histopathological alterations provide a good model fish and reliable early biomarkers for monitoring Cu pollution in seawater and might call for the protection agencies to revise the Cu environmental standards.
已经实现了一种综合方法来研究短期铜暴露对欧洲海鲈（Dicentrarchus labrax）幼鱼的化学积累和生物效应。鱼在 0.01-10mg/L 名义铜浓度下暴露 24-96 小时。研究了水中和鳃、肝脏、肌肉和脑组织中的金属浓度，以及氧化应激生物标志物（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、脂质过氧化）。在所有器官中都观察到了氧化损伤的诱导，同时也观察到了不同的抗氧化反应；在低环境水中铜浓度（0.01mg/L）时，鳃作为最敏感的器官出现。即使没有明显的铜积累，也观察到了肝脏和鳃的组织病理学改变。结果表明，氧化应激参数的组合，特别是脂质过氧化和谷胱甘肽过氧化物酶活性以及组织病理学改变，为监测海水中铜污染提供了良好的鱼类模型和可靠的早期生物标志物，并可能呼吁保护机构修订铜环境标准。
