Uma Anitha K P G, Mythili S
Department of Biotechnology, School of Bio Sciences and Technology, VIT University, Vellore 14, Tamil Nadu, India.
Department of Biotechnology, School of Bio Sciences and Technology, VIT University, Vellore 14, Tamil Nadu, India.
Asian Pac J Trop Med. 2017 Jun;10(6):588-593. doi: 10.1016/j.apjtm.2017.06.008. Epub 2017 Jun 15.
To isolate, identify and evaluate the antioxidant, antimicrobial, hepatoprotective potentials, total phenolic content, flavonoid content, tannin content of ethyl acetate extract of endophytic fungus Achaetomium sp., isolated from Euphorbia hirta.
Hepatoprotectivity of ethyl acetate extract of Achaetomium sp., was evaluated by CCl induced toxicity in HepG2 cells and subsequently analysed for cell viability using MTT assay. It also demonstrates antioxidant and antimicrobial potentials by DPPH radical scavenging assay and well diffusion assay respectively. Quantification of total phenolic content, tannin content and flavonoid content were assessed by spectroscopic methods.
Phenols, flavonoids and tannins were the phytochemicals present in ethyl acetate extract of Achaetomium sp., with rich phenolic content exhibited potent hepatoprotective, antimicrobial and antioxidant activities. The hepatoprotective activity was recorded as of 72.13% ± 2.948% of cell viability at a concentration of 150 μg/mL, whereas the standard silymarin showed 93.260% ± 0.784%. It was observed to be dose dependent, when CCl exposed HepG2 cells were treated with different concentrations of ethyl acetate extract. Antibacterial activity showed significant inhibition against Staphylococcus aureus, Pseudomonas aeroginosa, and Klebsiella pneumoniae. The antioxidant activity ranged from 66.890% ± 1.385% to 87.340% ± 0.289% with (44.02 ± 1.57) μg of total phenolics, (54.54 ± 1.82) μg of flavonoid content and (18.790 ± 1.018) μg of tannin content. Ascorbic acid, BHT (butylated hydroxyl toluene) Gallic acid and Pyrogallol were used as standards which showed 98.370% ± 0.763%; 97.080% ± 0.636%; 94.890% ± 1.103% and 96.980% ± 0.098% reducing potential respectively.
The results reveal that the metabolites produced by endophytic fungi isolated from Euphorbia hirta could be novel natural products that could lead to new drug discovery.
从大戟属植物大戟中分离、鉴定内生真菌曲霉属菌株的乙酸乙酯提取物，并评估其抗氧化、抗菌、保肝潜力、总酚含量、黄酮含量和单宁含量。
通过CCl诱导HepG2细胞毒性来评估曲霉属菌株乙酸乙酯提取物的保肝活性，随后使用MTT法分析细胞活力。它还分别通过DPPH自由基清除试验和滤纸片扩散法证明了其抗氧化和抗菌潜力。通过光谱法对总酚含量、单宁含量和黄酮含量进行定量分析。
酚类、黄酮类和单宁类是曲霉属菌株乙酸乙酯提取物中的植物化学物质，富含酚类物质的提取物表现出强大的保肝、抗菌和抗氧化活性。在浓度为150μg/mL时，保肝活性记录为细胞活力的72.13%±2.948%，而标准水飞蓟素显示为93.260%±0.784%。当用不同浓度的乙酸乙酯提取物处理CCl暴露的HepG2细胞时，观察到其具有剂量依赖性。抗菌活性对金黄色葡萄球菌、铜绿假单胞菌和肺炎克雷伯菌显示出显著抑制作用。抗氧化活性范围为66.890%±1.385%至87.340%±0.289%，总酚含量为(44.02±1.57)μg，黄酮含量为(54.54±1.82)μg，单宁含量为(18.790±1.018)μg。以抗坏血酸、丁基羟基甲苯（BHT）没食子酸和邻苯三酚作为标准品，其还原电位分别为98.370%±0.763%、97.080%±0.636%、94.890%±1.103%和96.980%±0.098%。
结果表明，从大戟属植物大戟中分离出的内生真菌产生的代谢产物可能是新型天然产物，有望用于新药研发。
