对一些苏丹药用植物内生真菌抗氧化潜力的初步研究。

A pilot study of antioxidant potential of endophytic fungi from some Sudanese medicinal plants.

作者信息

Khiralla Afra, Mohamed Ietidal, Thomas Justinne, Mignard Benoît, Spina Rosella, Yagi Sakina, Laurain-Mattar Dominique

机构信息

Université de Lorraine, SRSMC, UMR 7565, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France; CNRS, SRSMC, UMR 7565, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France; Botany Department, Faculty of Science, University of Khartoum, P.O. Box 321, Khartoum, Sudan.

Botany Department, Faculty of Science, University of Khartoum, P.O. Box 321, Khartoum, Sudan.

出版信息

Asian Pac J Trop Med. 2015 Sep;8(9):701-4. doi: 10.1016/j.apjtm.2015.07.032. Epub 2015 Jul 29.

DOI:10.1016/j.apjtm.2015.07.032

PMID:26433653

Abstract

OBJECTIVE

To evaluate the total phenolic content and total antioxidant capacity of ethyl acetate extracts of 21 endophytic fungi isolated from five Sudanese medicinal plants: Calotropis procera, Catharanthus roseus, Euphorbia prostrate, Vernonia amygdalina and Trigonella foenum-graecum.

METHODS

Crude extracts of endophytic fungi and their host plants were tested by classical Folin-Ciocalteu colorimetric method to determine the total phenolic content, also total antioxidant capacity was estimated using 1,1-diphenyl-2-picrylhydrazyl free radical scavenging in vitro method.

RESULTS

Among the endophytes, endophytic fungus Aspergillus sp. from Trigonella foenum-graecum seeds demonstrated the highest both total phenolic content in term of gallic acid equivalent [(89.9 ± 7.1) mg GAE/g] and antioxidant activity for 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay [IC50: (18.0 ± 0.1) μg/mL]. A high positive linear correlation (R(2) = 0.999 1) was found between total antioxidant capacity and total phenolic content of endophytic fungi isolated from Vernonia amygdalina.

CONCLUSIONS

The present study revealed that some endophytic fungi from the five Sudanese medicinal plants could be a potential source of novel natural antioxidant compounds.

摘要

目的

评估从5种苏丹药用植物（牛角瓜、长春花、匍匐大戟、扁桃斑鸠菊和胡芦巴）中分离出的21株内生真菌的乙酸乙酯提取物的总酚含量和总抗氧化能力。

方法

采用经典的福林-酚比色法检测内生真菌及其宿主植物的粗提物，以测定总酚含量，同时采用1,1-二苯基-2-苦基肼自由基体外清除法评估总抗氧化能力。

结果

在内生真菌中，从胡芦巴种子中分离出的内生真菌曲霉属表现出最高的总酚含量（以没食子酸当量计为[(89.9 ± 7.1) mg GAE/g]）以及1,1-二苯基-2-苦基肼自由基清除试验的抗氧化活性[IC50：(18.0 ± 0.1) μg/mL]。从扁桃斑鸠菊中分离出的内生真菌的总抗氧化能力与总酚含量之间存在高度正线性相关（R(2) = 0.999 1）。