Albayrak Sevil, Atasagun Bayram, Aksoy Ahmet
Erciyes University, Science Faculty, Department of Biology, 38039 Kayseri, Turkey.
Erciyes University, Science Faculty, Department of Biology, 38039 Kayseri, Turkey.
Asian Pac J Trop Med. 2017 Jun;10(6):599-606. doi: 10.1016/j.apjtm.2017.06.010. Epub 2017 Jun 15.
The present study aimed to compare the polyphenol content, total phenolic, total flavonoid, antioxidant and antimicrobial activity of the extracts obtained from Centaurea amaena Boiss. & Balansa and Centaurea aksoyi Hamzaoğlu & Budak.
Both species were subjected to maceration, Soxhlet and ultrasonication extractions with methanol in order to macerated (ME), Soxhlet (SE) and ultrasonicated (UE) extracts. Their phenolic profiles were qualitatively examined by LC-MS. Their antioxidant activities were determined by phosphomolybdenum, β-carotene bleaching and DPPH assays. Agar diffusion and broth dilution methods were carried out to find the antimicrobial activity of these extracts against fifteen microorganisms.
Quercetin, quercetin-3-β-D-glucoside and protocatechuic acid were the main components of the both extracts obtained by Soxhlet extraction. The highest phenolic and flavonoid contents were found in the UE for both species. All the extracts exhibited good total antioxidant and DPPH radical scavenging activity. UE obtained from C. amaena showed the highest antioxidant activity with the highest phenolic and flavonoid contents. The antibacterial activity of UE obtained from C. amaena was better than other extracts and antibacterial activity of C. amaena was also better than C. aksoyi.
This study confirms that ultrasonic extraction may be an ideal, simple and rapid method to obtain polyphenol-rich extracts have antioxidant as well as antibacterial activity from both Centaurea species especially from C. amaena.
本研究旨在比较紫苞矢车菊（Centaurea amaena Boiss. & Balansa）和阿克索伊矢车菊（Centaurea aksoyi Hamzaoğlu & Budak）提取物的多酚含量、总酚、总黄酮、抗氧化和抗菌活性。
对这两个物种进行浸渍、索氏提取和超声提取，以获得浸渍提取物（ME）、索氏提取物（SE）和超声提取物（UE），提取溶剂为甲醇。通过液相色谱-质谱联用（LC-MS）对其酚类成分进行定性分析。通过磷钼酸法、β-胡萝卜素漂白法和二苯基苦味酰基自由基（DPPH）法测定其抗氧化活性。采用琼脂扩散法和肉汤稀释法来检测这些提取物对15种微生物的抗菌活性。
槲皮素、槲皮素-3-β-D-葡萄糖苷和原儿茶酸是索氏提取得到的两种提取物的主要成分。两种植物的超声提取物中酚类和黄酮类含量最高。所有提取物均表现出良好的总抗氧化活性和DPPH自由基清除活性。紫苞矢车菊的超声提取物具有最高的抗氧化活性，酚类和黄酮类含量也最高。紫苞矢车菊的超声提取物的抗菌活性优于其他提取物，且紫苞矢车菊的抗菌活性也优于阿克索伊矢车菊。
本研究证实，超声提取可能是一种理想、简单且快速的方法，可从这两种矢车菊属植物，尤其是紫苞矢车菊中获得具有抗氧化和抗菌活性的富含多酚的提取物。
