扩展卫星重复扩增了驱动雌性减数分裂的染色体上离散的 CENP-A 核小体组装位点。
Expanded Satellite Repeats Amplify a Discrete CENP-A Nucleosome Assembly Site on Chromosomes that Drive in Female Meiosis.
机构信息
Department of Biology, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA 19104, USA.
Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
出版信息
Curr Biol. 2017 Aug 7;27(15):2365-2373.e8. doi: 10.1016/j.cub.2017.06.069. Epub 2017 Jul 27.
Abstract
Female meiosis provides an opportunity for selfish genetic elements to violate Mendel's law of segregation by increasing the chance of segregating to the egg [1]. Centromeres and other repetitive sequences can drive in meiosis by cheating the segregation process [2], but the underlying mechanisms are unknown. Here, we show that centromeres with more satellite repeats house more nucleosomes that confer centromere identity, containing the histone H3 variant CENP-A, and bias their segregation to the egg relative to centromeres with fewer repeats. CENP-A nucleosomes predominantly occupy a single site within the repeating unit that becomes limiting for centromere assembly on smaller centromeres. We propose that amplified repetitive sequences act as selfish elements by promoting expansion of CENP-A chromatin and increased transmission through the female germline.
摘要
女性减数分裂为自私的遗传元件提供了一个机会，通过增加分配到卵子的几率，违反孟德尔的分离定律[1]。着丝粒和其他重复序列可以通过欺骗分离过程[2]在减数分裂中驱动，但潜在的机制尚不清楚。在这里，我们表明，具有更多卫星重复的着丝粒拥有更多的核小体，赋予着丝粒身份，包含组蛋白 H3 变体 CENP-A，并使它们相对于具有较少重复的着丝粒偏向于分配到卵子中。CENP-A 核小体主要占据重复单元内的一个单一位置，该位置对于较小的着丝粒上的着丝粒组装成为限制因素。我们提出，扩增的重复序列通过促进 CENP-A 染色质的扩展和通过雌性生殖系的增加传递，充当自私的元件。
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本文引用的文献
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