Bengtsson Björn, Persson Lotta, Ekström Kerstin, Unnerstad Helle Ericsson, Uhlhorn Henrik, Börjesson Stefan
National Veterinary Institute (SVA), Sweden.
Swedish Animal Welfare Association, Sweden.
Vet Microbiol. 2017 Aug;207:103-107. doi: 10.1016/j.vetmic.2017.06.004. Epub 2017 Jun 6.
We investigated the occurrence of mecC-MRSA in wild hedgehogs (Erinaceus europaeus) in Sweden and characterized the obtained isolates. Samples from 55 hedgehogs from five counties of Sweden were cultivated selectively for MRSA and putative isolates were confirmed by real-time PCR detecting mecA, mecC, nuc and PVL genes. mecC-MRSA was confirmed in 35 (64%) animals from three geographically separated counties. Confirmed isolates were spa-typed and tested for antimicrobial susceptibility by broth microdilution. Eight different spa-types were identified (t843, t978, t3391, t9111, t10751, t10893, t11015, t15312) of which t843 (49%) was the most common. The spa-types t843, t3391 and t978 were found in isolates from two counties. The study shows that mecC-MRSA is common in wild hedgehogs in two counties of Sweden but occurs in hedgehogs also in other parts of the country. Our findings suggest that hedgehogs could be a reservoir for mecC-MRSA. In addition, similar spa-types of isolates from hedgehogs and isolates previously described in domesticated animals and in humans indicates transfer between these populations.
我们调查了瑞典野生刺猬（欧洲刺猬）中耐甲氧西林金黄色葡萄球菌（mecC-MRSA）的出现情况，并对分离得到的菌株进行了特征分析。从瑞典五个县的55只刺猬采集样本，选择性培养耐甲氧西林金黄色葡萄球菌，通过实时聚合酶链反应检测mecA、mecC、nuc和杀白细胞素（PVL）基因来确认假定的分离株。在来自三个地理上分离的县的35只（64%）动物中确认存在mecC-MRSA。对确认的分离株进行葡萄球菌蛋白A（spa）分型，并通过肉汤微量稀释法检测其抗菌药敏性。鉴定出八种不同的spa型（t843、t978、t3391、t9111、t10751、t10893、t11015、t15312），其中t843（49%）最为常见。spa型t843、t3391和t978在来自两个县的分离株中被发现。该研究表明，mecC-MRSA在瑞典两个县的野生刺猬中很常见，但在该国其他地区的刺猬中也有出现。我们的研究结果表明，刺猬可能是mecC-MRSA的储存宿主。此外，刺猬分离株与先前在家养动物和人类中描述的分离株具有相似的spa型，这表明这些种群之间存在转移。
