Foster Shadae R, Dilworth Lowell L, Thompson Rory K, Alexander-Lindo Ruby L, Omoruyi Felix O
Department of Basic Medical Sciences, Biochemistry Section, UWI, Mona, Jamaica.
Department of Pathology, UWI, Mona, Jamaica.
Chem Biol Interact. 2017 Sep 25;275:108-115. doi: 10.1016/j.cbi.2017.07.024. Epub 2017 Jul 28.
Diabetes mellitus is associated with elevated reactive oxygen species, lipid abnormalities, reduced antioxidant activity and organ damage. This study examines the effects of combined inositol hexakisphosphate (IP6) and inositol supplement on antioxidant levels and other biochemical parameters in the liver of type 2 diabetic rats. Five groups of Sprague-Dawley rats were studied. Six rats were fed normal diet (non-diabetic control), while 24 rats were fed high-fat diet (HFD) for 4 weeks. Diabetes was induced in 18 of the rats fed HFD by intraperitoneal administration of streptozotocin. The diabetic rats were separated into three groups namely: combined IP6 and inositol, glibenclamide and diabetic control. The non-diabetic group fed high-fat diet was classified as a high-fat control group. For the final four weeks of the experiment, all rats were fed normal diet and given their respective treatment regimes. Hepatic antioxidant status, metabolic enzyme activity, lipid profile, peroxidative damage and liver histology, as well as, serum aminotransferase and alkaline phosphatase activities, and total bilirubin concentration were assessed. Treatment with combined IP6 and inositol supplement significantly increased liver reduced glutathione and high-density lipoprotein levels while liver triglyceride levels and serum alkaline phosphatase activity were significantly reduced by 27%, 50%, 38.5%, and 69.2% respectively compared to the diabetic control. Hepatic superoxide dismutase, catalase, glucose-6-phosphate dehydrogenase activities were significantly upregulated by 55%, 26% and 53% respectively in the diabetic rats treated with combined IP6 and inositol compared to the diabetic control. Combined IP6 and inositol treatment resulted in the preservation of liver cell integrity and improved antioxidant status in type 2 diabetic rats.
糖尿病与活性氧升高、脂质异常、抗氧化活性降低及器官损伤有关。本研究考察了联合使用肌醇六磷酸（IP6）和肌醇补充剂对2型糖尿病大鼠肝脏抗氧化水平及其他生化参数的影响。研究了五组斯普拉格-道利大鼠。六只大鼠喂食正常饮食（非糖尿病对照组），而24只大鼠喂食高脂饮食（HFD）4周。通过腹腔注射链脲佐菌素，在18只喂食HFD的大鼠中诱导糖尿病。将糖尿病大鼠分为三组，即：联合IP6和肌醇组、格列本脲组和糖尿病对照组。喂食高脂饮食的非糖尿病组被归类为高脂对照组。在实验的最后四周，所有大鼠均喂食正常饮食并给予各自的治疗方案。评估肝脏抗氧化状态、代谢酶活性、血脂谱、过氧化损伤和肝脏组织学，以及血清转氨酶和碱性磷酸酶活性和总胆红素浓度。与糖尿病对照组相比，联合使用IP6和肌醇补充剂治疗显著提高了肝脏还原型谷胱甘肽和高密度脂蛋白水平，同时肝脏甘油三酯水平和血清碱性磷酸酶活性分别显著降低了27%、50%、38.5%和69.2%。与糖尿病对照组相比，联合使用IP6和肌醇治疗的糖尿病大鼠肝脏超氧化物歧化酶、过氧化氢酶、葡萄糖-6-磷酸脱氢酶活性分别显著上调了55%、26%和53%。联合使用IP6和肌醇治疗可维持2型糖尿病大鼠肝细胞完整性并改善抗氧化状态。
