Kuppusamy Asokkumar, Muthusamy Umamaheswari, Thirumalaisamy Sivashanmugam Andichetiar, Varadharajan Subhadradevi, Ramasamy Kalyanasubramaniam, Ramanathan Sambathkumar
University of Al-Jabal Al-Gharbi.
J Complement Integr Med. 2011 Jan;8. doi: 10.2202/1553-3840.1483.
Phytic acid, inositol hexaphosphate (IP6) a natural plant constituent and antioxidant exhibits protective action in carcinogenesis, Alzheimer's, hypercholesterolemia, diabetes and inflammations when taken in diet. The aim of this study is to evaluate effect of phytic acid in streptozotocin (STZ)-nicotinamide-induced type 2 diabetes in rats. The STZ-nicotinamide induced diabetic rats were orally treated with vehicle (2%w/v Tween 80), glimepiride (2.5 mg/kg) and IP6 (650 mg/kg) for 28 days. The blood glucose level, body weight, glycosylated haemoglobin (HbA1C), lipid profile, lipid peroxidation, antioxidant status (liver and small intestine) was measured and compared with control. Xanthine dehydrogenase (XDH) and xanthine (XO) activity was measured in small intestine of diabetic rats. In vitro inhibition of carbohydrate digestive enzymes (α-glucosidase and α-amylase) was also determined. IP6, significantly (P<0.01) reduced glucose level, HbA1C, lipid profile and lipid peroxidation, and increased body weight, high density lipoprotein level and antioxidant status in liver and small intestine. Decrease in XO and increase in XDH activity was observed in treatment groups compared to diabetic control. Dose dependent inhibition of α-glucosidase and (α-amylase activity was observed for phytic acid when compared to standard drug acarbose. These results clearly indicate that IP6 possess promising in vitro and in vivo antidiabetic activity.
植酸，即肌醇六磷酸（IP6），是一种天然植物成分和抗氧化剂，在饮食中摄入时对癌症、阿尔茨海默病、高胆固醇血症、糖尿病和炎症具有保护作用。本研究的目的是评估植酸对链脲佐菌素（STZ）-烟酰胺诱导的大鼠2型糖尿病的影响。将STZ-烟酰胺诱导的糖尿病大鼠口服给予赋形剂（2%w/v吐温80）、格列美脲（2.5mg/kg）和IP6（650mg/kg），持续28天。测量血糖水平、体重、糖化血红蛋白（HbA1C）、血脂谱、脂质过氧化、抗氧化状态（肝脏和小肠），并与对照组进行比较。测定糖尿病大鼠小肠中的黄嘌呤脱氢酶（XDH）和黄嘌呤氧化酶（XO）活性。还测定了碳水化合物消化酶（α-葡萄糖苷酶和α-淀粉酶）的体外抑制作用。IP6显著（P<0.01）降低了血糖水平、HbA1C、血脂谱和脂质过氧化，并增加了体重、高密度脂蛋白水平以及肝脏和小肠中的抗氧化状态。与糖尿病对照组相比，治疗组XO活性降低，XDH活性增加。与标准药物阿卡波糖相比，观察到植酸对α-葡萄糖苷酶和α-淀粉酶活性具有剂量依赖性抑制作用。这些结果清楚地表明，IP6具有良好的体外和体内抗糖尿病活性。
