肠杆菌科 cloacae ST520 的草图基因组序列，该菌携带 blaCTX-M-15、blaDHA-1 和 blaOXA-48 基因，从南大西洋海域的沿海水域中分离得到。
Draft genome sequence of Enterobacter cloacae ST520 harbouring bla, bla and bla isolated from coastal waters of the South Atlantic Ocean.
机构信息
Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
出版信息
J Glob Antimicrob Resist. 2017 Sep;10:279-280. doi: 10.1016/j.jgar.2017.07.017. Epub 2017 Aug 4.
OBJECTIVES
Aquatic environments have contributed to the dissemination of multidrug-resistant (MDR) bacteria, representing a risk for humans and animals. The aim of this study was to report the first draft genome sequence of a MDR Enterobacter cloacae strain recovered from seawater in a public beach in Brazil.
METHODS
The genome was sequenced on an Illumina MiSeq platform. De novo genome assembly was performed using SPAdes 3.10.1 and the whole genome sequence was analysed using bioinformatics tools from the Center of Genomic Epidemiology.
RESULTS
This draft genome resulted in 5 228 857bp with 5331 protein-coding sequences, revealing the presence of bla, bla and bla genes, responsible for resistance to all β-lactam antibiotics. In addition, the strain was assigned to sequenced type 520 (ST520).
CONCLUSION
These data provide useful information for comparative genomic analysis regarding the dissemination of antibiotic resistance genes.
目的
水生环境促进了多重耐药（MDR）细菌的传播，对人类和动物构成了威胁。本研究旨在报告从巴西一个公共海滩海水中分离出的一株 MDR 阴沟肠杆菌的首个基因组草图序列。
方法
使用 Illumina MiSeq 平台对基因组进行测序。使用 SPAdes 3.10.1 进行从头基因组组装，使用基因组流行病学中心的生物信息学工具对全基因组序列进行分析。
结果
该草图基因组共 5228857bp，包含 5331 个蛋白质编码序列，显示存在 blaCTX-M-15、blaDHA-1 和 blaOXA-10 基因，对所有β-内酰胺类抗生素均有耐药性。此外，该菌株被分配到测序型 520（ST520）。
结论
这些数据为抗生素耐药基因传播的比较基因组分析提供了有用的信息。
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