Ranjbar Reza, Arjomandzadegan Mohammad, Hosseiny Hossein
Molecular Biology Research Center, Baqiyatallah University of MedicalSciences, 1417613151 Tehran, Iran.
Infectious Diseases Research Center (IDRC) and Department of Microbiology, School of Medicine, Arak University of Medical Sciences, 3813898197 Arak, Iran.
Sci Pharm. 2017 Jul 31;85(3):28. doi: 10.3390/scipharm85030028.
The aim of the study was to examine antibacterial properties of microemulsion structure produced from var. extract as a new tool of nanoscale drug-like materials. var. () extract was prepared by distillation method. A nonocarrier structure in the microemulsion system was prepared from the extract. Serial concentrations were prepared from 8 mg/mL extract and the nonocarrier containing 0.1 mg/mL pure extract and were evaluated by a disk diffusion method for 35 clinical isolates. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by microbroth dilution assay using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method by an enzyme-linked immunosorbent assay(ELISA) Microplate Reader apparatus. Antioxidant activity of the extract was determined by measuring the ferric reducing ability of plasma (FRAP) assay. From 35 clinical isolates of , 17 isolates-including resistant isolates of S.E.1103 and S.E.49-had a zone of inhibition (ZI) of 7 to 32 mm in 0.007 mg/mL of the extract. S.E.76 isolate exposed to 30 µg/mL ceftazidime disk had a ZI of 12 mm but had 10 mm in 7µg/mL of extract. The inhibitory effect of a nanocarrier at a concentration of 25 µg/mL by 20 mm ZI was comparable by the ceftazidime (30 µg/mL) effect. MIC was 0.25 mg/mL and MBC was 0.5 mg/mL by MTT method for the extract. It was shown that extract had antioxidant activity of 31.67 µM/mg that could be increased based on concentration. It was concluded that the nanocarrier had a significant effect on the studied isolates in comparison with ordinary antibiotics and had potential for use as a natural antioxidant and antimicrobial material in complementary medicine.
本研究的目的是检测由变种提取物制备的微乳液结构作为一种新型纳米级类药物材料的抗菌性能。变种（）提取物通过蒸馏法制备。从该提取物制备微乳液体系中的非载体结构。从8mg/mL提取物和含有0.1mg/mL纯提取物的非载体中制备系列浓度，并通过纸片扩散法对35株临床分离株进行评估。使用MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法通过酶联免疫吸附测定（ELISA）微孔板读数仪装置，通过微量肉汤稀释法测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。通过测量血浆铁还原能力（FRAP）测定法确定提取物的抗氧化活性。在35株临床分离株中，17株分离株——包括S.E.1103和S.E.49的耐药分离株——在0.007mg/mL提取物中抑菌圈（ZI）为7至32mm。暴露于30μg/mL头孢他啶纸片的S.E.76分离株抑菌圈为12mm，但在7μg/mL提取物中为10mm。浓度为25μg/mL的纳米载体通过20mm抑菌圈产生的抑制作用与头孢他啶（30μg/mL）的作用相当。通过MTT法测定提取物的MIC为0.25mg/mL，MBC为0.5mg/mL。结果表明，提取物具有31.67μM/mg的抗氧化活性，且该活性可随浓度增加。得出的结论是，与普通抗生素相比，该纳米载体对所研究的分离株有显著影响，并且在补充医学中具有作为天然抗氧化剂和抗菌材料使用的潜力。
