Abdelkarem Hala M, Fadda Laila H, El-Sayed Eman M, Radwan Omyma K
a Nutrition Department , National Research Center , Dokki , Cairo , Egypt.
b Faculty of Pharmacy, Pharmaceutical Department , King Saud University , Riyadh , Saudi Arabia.
J Diet Suppl. 2018 May 4;15(3):300-310. doi: 10.1080/19390211.2017.1343889. Epub 2017 Jul 31.
The purpose of this study was to illustrate the effects of zinc oxide nanoparticles (ZnO-NPs) administration on bone turnover and bone resorbing agents in rats and how L-arginine (L-arg) or vitamin E (vit E) co-administrations might affect them. Fasting rats were randomly divided into four groups (n = 10): G1-normal healthy animals; G2-ZnO-NPs-exposed rats (600 mg/kg1/day); G3-ZnO-NPs-exposed rats co-administrated L-arg (200 mg/kg1/day); G4-ZnO-NPs-exposed rats co-administrated vit E (200 mg/kg1/day). The ingredients were orally administered daily. The body weight and food consumption of rats were recorded during the administration period and the experiment continued for three consecutive weeks. The results demonstrated that ZnO-NPs administration induced bone loss in rats as manifested by reduced activity of bone alkaline phosphatase (B-ALP) and increased level of C-terminal peptide type I collagen (CTx). The increase of inflammatory markers, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) by ZnO-NPs suggests that deleterious effects of ZnO-NPs on bone turnover were, in part, due to inflammation. Confirming to this suggestion, both L-arg and vit E reduced TNF-α and IL-6 levels and consequently decreased bone resorption as indicated by reduced serum CTx level. This study proved that ZnO-NPs can induce bone turnover, which may be reduced by L-arg or vit.E co-administration, partly by anti-inflammatory mechanism.
本研究的目的是阐明给予大鼠氧化锌纳米颗粒（ZnO-NPs）对骨转换和骨吸收因子的影响，以及联合给予L-精氨酸（L-arg）或维生素E（vit E）会如何影响它们。将禁食的大鼠随机分为四组（n = 10）：G1组为正常健康动物；G2组为暴露于ZnO-NPs的大鼠（600 mg/kg1/天）；G3组为联合给予L-arg（200 mg/kg1/天）的暴露于ZnO-NPs的大鼠；G4组为联合给予vit E（200 mg/kg1/天）的暴露于ZnO-NPs的大鼠。每天经口给予这些成分。在给药期间记录大鼠的体重和食物摄入量，实验持续三周。结果表明，给予ZnO-NPs可导致大鼠骨量流失，表现为骨碱性磷酸酶（B-ALP）活性降低和I型胶原C末端肽（CTx）水平升高。ZnO-NPs使炎症标志物肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）升高，这表明ZnO-NPs对骨转换的有害作用部分归因于炎症。与此观点相符的是，L-arg和vit E均降低了TNF-α和IL-6水平，从而降低了血清CTx水平所表明的骨吸收。本研究证明，ZnO-NPs可诱导骨转换，联合给予L-arg或vit E可部分通过抗炎机制降低这种作用。
