Saki Forough, Rahimikashkooli Nima, Masjedi Moein, Dastghaib Sanaz, Koohpeyma Farhad
Pediatric Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, P.O. Box, Shiraz, 71345-1744, Iran.
Internal Medicine Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
BMC Complement Med Ther. 2024 Dec 26;24(1):425. doi: 10.1186/s12906-024-04736-3.
L-arginine (Arg) is a semi-essential amino acid that can be used as a key mediator for the release of growth hormone (GH), insulin-like growth factor-1(IGF-1), and other growth factors. In this study, we comprehensively evaluated the effect of Arg intake on bone growth and associated markers.
The study involved 24 Sprague-Dawley rats (12 males, 12 females) divided into two groups (Age = 24 days). One group received a standard diet, while the other was injected with 10 mg/kg of Arg daily for 90 days. Serum bone markers like calcium (Ca), phosphorous(P), and alkaline phosphatase (ALP) were analyzed via colorimetric assays. stereological study and bone mineral density (BMD) were conducted via dissector method and Hologic Dual-energy x-ray absorptiometry (DXA) system; respectively.
Biochemical assays showed no significant differences in Ca, P, and ALP levels between groups. Male rats in the case group exhibited lower testosterone levels (p.value = 0.009). Stereological and bone mineral density (BMD) analyses revealed contrasting gender-specific outcomes. Female rats in the case group had higher BMD (p.value = 0.001), while males had lower BMD compared to controls (p.value = 0.018). Arg consumption affects trabecula volume values differently in females compared to males (p.value = 0.022). Furthermore, the study observed decreased osteocytes and osteoblasts in male case rats. The gender-based differences in BMD were attributed to Arg's paradoxical impact on testosterone levels in males.
Overall, Arg supplementation was found to influence BMD and trabecular bone volume, with outcomes varying depending on gender. The study highlights the intricate interplay between Arg, sex hormones, and bone health, offering insights into these complex relationships.
L-精氨酸（Arg）是一种半必需氨基酸，可用作生长激素（GH）、胰岛素样生长因子-1（IGF-1）及其他生长因子释放的关键介质。在本研究中，我们全面评估了摄入精氨酸对骨骼生长及相关标志物的影响。
本研究纳入24只斯普拉格-道利大鼠（12只雄性，12只雌性），分为两组（年龄 = 24天）。一组给予标准饮食，另一组每天注射10 mg/kg精氨酸，持续90天。通过比色法分析血清骨标志物，如钙（Ca）、磷（P）和碱性磷酸酶（ALP）。分别通过分割法和Hologic双能X线吸收法（DXA）系统进行体视学研究和骨密度（BMD）测定。
生化分析显示，两组之间的钙、磷和碱性磷酸酶水平无显著差异。病例组雄性大鼠的睾酮水平较低（p值 = 0.009）。体视学和骨密度分析显示出不同性别的对比结果。病例组雌性大鼠的骨密度较高（p值 = 0.001），而雄性大鼠的骨密度低于对照组（p值 = 0.018）。与雄性相比，精氨酸摄入对雌性小梁体积值的影响不同（p值 = 0.022）。此外，研究观察到病例组雄性大鼠的骨细胞和成骨细胞减少。骨密度的性别差异归因于精氨酸对雄性睾酮水平的矛盾影响。
总体而言，发现补充精氨酸会影响骨密度和小梁骨体积，结果因性别而异。该研究突出了精氨酸、性激素和骨骼健康之间的复杂相互作用，为这些复杂关系提供了见解。
