Ding Zhou, Jiang Changjun
School of Tea and Food Science Technology, Anhui Agricultural University, Hefei, China.
State Key Laboratory of Tea Biology and Utilization, Anhui Agricultural University, Hefei, China.
Front Genet. 2022 Aug 10;13:907026. doi: 10.3389/fgene.2022.907026. eCollection 2022.
Tea plant () is an important economic beverage crop. Drought stress seriously affects the growth and development of tea plant and the accumulation of metabolites, as well as the production, processing, yield and quality of tea. Therefore, it is necessary to understand the reaction mechanism of tea plant under drought conditions and find efficient control methods. Based on transcriptome sequencing technology, this study studied the difference of metabolic level between sexual and asexual tea plants under drought stress. In this study, there were multiple levels of up-regulation and down-regulation of differential genes related to cell composition, molecular function and biological processes. Transcriptomic data show that the metabolism of tea plants with different propagation modes of QC and ZZ is different under drought conditions. In the expression difference statistics, it can be seen that the differential genes of QC are significantly more than ZZ; GO enrichment analysis also found that although differential genes in biological process are mainly enriched in the three pathways of metabolic, single organism process and cellular process, cellular component is mainly enriched in cell, cell part, membrane, and molecular function, and binding, catalytic activity, and transporter activity; the enrichment order of differential genes in these pathways is different in QC and ZZ. This difference is caused by the way of reproduction. The further study of these differential genes will lay a foundation for the cultivation methods and biotechnology breeding to improve the quality of tea.
茶树()是一种重要的经济饮料作物。干旱胁迫严重影响茶树的生长发育、代谢产物积累以及茶叶的生产、加工、产量和品质。因此,有必要了解茶树在干旱条件下的反应机制并找到有效的控制方法。基于转录组测序技术,本研究探讨了干旱胁迫下有性和无性茶树代谢水平的差异。本研究中,与细胞组成、分子功能和生物学过程相关的差异基因存在多个上调和下调水平。转录组数据表明,干旱条件下,具有不同繁殖模式的QC和ZZ茶树的代谢情况不同。在表达差异统计中可以看出,QC的差异基因明显多于ZZ;GO富集分析还发现,虽然生物学过程中的差异基因主要富集在代谢、单生物体过程和细胞过程这三个途径中,但细胞组分主要富集在细胞、细胞部分、膜中,分子功能主要富集在结合、催化活性和转运活性中;这些途径中差异基因的富集顺序在QC和ZZ中不同。这种差异是由繁殖方式造成的。对这些差异基因的进一步研究将为提高茶叶品质的栽培方法和生物技术育种奠定基础。