Adiponectin Upregulates Expression via SIRT1-Mediated Signaling in Human Normal Keratinocytes.

BACKGROUND

() is the major component of the epidermal granular layer and binds to and condenses the keratin cytoskeleton. thus contributes to cell compaction and serves as a natural moisturizing factor by promoting unfolding and degradation into hygroscopic amino acids. Loss or downregulation of has been shown to result in a weak stratum corneum, which causes water loss and increases the possibility of skin barrier-related seizure. Adiponectin (Acrp30) contributes to the functional recovery of somatic cells, including human normal epidermal keratinocytes (NHEKs).

OBJECTIVE

To investigate the effect of Acrp30 in expression and identifying its signal transduction mechanism.

METHODS

Normal human keratinocytes were treated with Acrp30 and the levels of were examined. Silent mating type information regulation 2 homolog (SIRT)-targeting siRNA and aryl hydrocarbon receptor nuclear translocator (ARNT)-targeting siRNA were used to identify the role of various signal transduction pathway components.

RESULTS

Acrp30 upregulated SIRT1 and ARNT expression in NHEKs, resulting in increased expression. Treatment with both SIRT1-targeting siRNA and ARNT-targeting siRNA blocked Acrp30 stimulation and silenced expression.

CONCLUSION

Adiponectin upregulates expression through a SIRT1-mediated pathway. Our results suggest that Acrp30 is a promising agent for skin barrier permeability improvement.