Mashwal Fahad A, El Safi Salah H, George Siju K, Adam Ahmed A, Jebakumar Arulanantham Z
Clinical Laboratory Science Department, Prince Sultan Military College of Health Sciences, Dhahran, Kingdom of Saudi Arabia. E-mail.
Saudi Med J. 2017 Jul;38(8):811-815. doi: 10.15537/smj.2017.8.18578.
To find the prevalence of uropathogenic Escherichia coli (E. coli)-producing extended spectrum beta lactamase (ESBL) at King Fahd Military Medical Complex in Dhahran (KFMMC) and to detect the genes responsible for its production. In addition, we determined the pattern of multi-drug resistance among isolates. Methods: A total of 117 uropathogenic E. coli isolates were collected from KFMMC over a period of 4 months from March 2014 to June 2014. These were received in the Microbiology Laboratory at Prince Sultan Military College of Health Sciences (PSMCHS), Dhahran, Saudi Arabia for analysis. The isolates were screened for ESBL using VITEK® 2 Compact. Polymerase chain reaction (PCR) examination was used to determine TEM, SHV, and CTX-M genes. Results: Our findings indicated that there is a high incidence of ESBLs among the E. coli isolated from UTI (23.1%). Our study also indicated that CTX-M genes are the most prevalent among the isolates at KFMMC followed by TEM class (6%), but there was also a higher percentage E. coli (3.4%) simultaneously harboring TEM and CTX genes. None of our isolates harbored the SHV genes. Conclusion: The findings document the threat of ESBL among E. coli isolates from UTI especially the CTX-M class in our hospital with the occurrence of these strains as etiologic agents of infection in the hospital and community.
为了确定达兰法赫德国王军事医疗中心（KFMMC）产超广谱β-内酰胺酶（ESBL）的尿路致病性大肠杆菌（E. coli）的流行情况，并检测其产生的相关基因。此外，我们还确定了分离株中的多重耐药模式。方法：在2014年3月至2014年6月的4个月期间，从KFMMC共收集了117株尿路致病性大肠杆菌分离株。这些分离株被送至沙特阿拉伯达兰苏丹王子军事健康科学学院（PSMCHS）的微生物实验室进行分析。使用VITEK® 2 Compact对分离株进行ESBL筛选。采用聚合酶链反应（PCR）检测TEM、SHV和CTX-M基因。结果：我们的研究结果表明，从尿路感染分离出的大肠杆菌中ESBLs的发生率很高（23.1%）。我们的研究还表明，CTX-M基因在KFMMC的分离株中最为普遍，其次是TEM类（6%），但同时携带TEM和CTX基因的大肠杆菌比例也较高（3.4%）。我们的分离株均未携带SHV基因。结论：研究结果证明了尿路感染大肠杆菌分离株中ESBL的威胁，尤其是我院的CTX-M类，这些菌株在医院和社区中作为感染病原体出现。
