默克尔细胞多瘤病毒（MCPyV）大T抗原在默克尔细胞癌淋巴结转移灶中的表达预示着预后不良。
Expression of Merkelcell polyomavirus (MCPyV) large T-antigen in Merkel cell carcinoma lymph node metastases predicts poor outcome.
作者信息
Haymerle Georg, Janik Stefan, Fochtmann Alexandra, Pammer Johannes, Schachner Helga, Nemec Lucas, Mildner Michael, Houben Roland, Grasl Matthaeus Ch, Erovic Boban M
机构信息
Department of Otolaryngology Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Department of Surgery, Clinical Division of Plastic and Reconstructive Surgery, Medical University of Vienna, Vienna, Austria.
出版信息
PLoS One. 2017 Aug 1;12(8):e0180426. doi: 10.1371/journal.pone.0180426. eCollection 2017.
BACKGROUND
The aim of this study was to determine the prevalence of MCPyV in Merkel cell carcinoma (MCC) primaries versus lymph node metastasis and to evaluate possible prognostic factors.
METHODS
Samples of MCC primaries and lymph node metastases were stained immunohistochemically for the MCPyV large T-antigen and expression was compared to patients´ clinical outcome.
RESULTS
41 MCC patients were included. 33 (61%) out of 54 specimens were MCPyV-positive in the immunohistochemistry. 15 (47%) out of 32 primary tumors were positive compared to 18 (82%) out of 22 lymph node metastases. Eleven patients with positive polyomavirus expression died from the carcinoma compared to 4 patients without virus expression. Cox regression analysis showed worse disease-free survival in patients with MCPyV compared to virus-negative lymph nodes (p = 0.002).
CONCLUSIONS
To our knowledge this is the first study to describe a negative prognostic effect of the MCPyV expression in lymph node metastasis in MCC patients.
背景
本研究的目的是确定 Merkel 细胞癌（MCC）原发灶与淋巴结转移中 Merkel 细胞多瘤病毒（MCPyV）的患病率，并评估可能的预后因素。
方法
对 MCC 原发灶和淋巴结转移灶样本进行免疫组织化学染色，检测 MCPyV 大 T 抗原，并将表达情况与患者的临床结局进行比较。
结果
纳入 41 例 MCC 患者。54 个标本中有 33 个（61%）在免疫组织化学中呈 MCPyV 阳性。32 个原发性肿瘤中有 15 个（47%）呈阳性，而 22 个淋巴结转移灶中有 18 个（82%）呈阳性。11 例多瘤病毒表达阳性的患者死于该癌症，而 4 例无病毒表达的患者死亡。Cox 回归分析显示，与病毒阴性淋巴结的患者相比，MCPyV 阳性患者的无病生存期更差（p = 0.002）。
结论
据我们所知，这是第一项描述 MCPyV 表达对 MCC 患者淋巴结转移具有负面预后影响的研究。
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