Kambale Pankaj Ramrao, Haldar Deepa, Kabi B C, Kambale Kalpana Pankaj
Assistant Professor, Department of Biochemistry, S.M.B.T. Institute of Medical Sciences and Research Centre, Nashik, Maharashtra, India.
Senior Resident, Department of Biochemistry, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India.
J Clin Diagn Res. 2017 Jun;11(6):BC05-BC08. doi: 10.7860/JCDR/2017/24290.9976. Epub 2017 Jun 1.
Incidence of prostate cancer is rising worldwide. Multiple factors have been suggested for the aetiology of prostate cancer including ethnic, genetic and diet. Vitamin D (calcitriol) has been shown to have role in cell growth and differentiation and its deficiency is implicated as one of the aetiological factors in prostate cancer. Prostatic epithelial cells express Vitamin D Receptor (VDR) as well as 1α- hydroxylase enzyme that are required for the synthesis of calcitriol and its action. Polymorphism in VDR gene has been associated with prostate cancer in some epidemiological studies; but, there is paucity of information in the Indian context.
The present study was aimed to explore the association of VDR gene polymorphism with the development of prostate cancer.
Three Single Nucleotide Polymorphisms (SNP) sites viz., FokI, TaqI and ApaI were analysed in 120 cases of prostate cancer which were compared with their 120 healthy first degree relatives and 120 non-related controls in the Department of Biochemistry in collaboration with the Department of Urology.
Analysis showed significantly decreased incidence of Tt and Aa genotype in prostate cancer patients as compared to healthy non-relative controls (p=0.016 and 0.043 respectively). As compared to first degree relatives, incidence of Tt genotype is significantly lower in cases (p=0.005). No significant association was found with FokI polymorphism.
This study suggests the protective role of heterozygous genotypes of TaqI and ApaI polymorphism against the development of prostate cancer.
全球范围内前列腺癌的发病率正在上升。前列腺癌的病因有多种因素，包括种族、遗传和饮食等。维生素D（骨化三醇）已被证明在细胞生长和分化中起作用，其缺乏被认为是前列腺癌的病因之一。前列腺上皮细胞表达维生素D受体（VDR）以及骨化三醇合成及其作用所需的1α-羟化酶。在一些流行病学研究中，VDR基因多态性与前列腺癌有关；但是，在印度背景下这方面的信息较少。
本研究旨在探讨VDR基因多态性与前列腺癌发生之间的关联。
在生物化学系与泌尿外科合作，对120例前列腺癌患者及其120名健康的一级亲属和120名无关对照进行了三个单核苷酸多态性（SNP）位点即FokI、TaqI和ApaI的分析。
分析显示，与健康的非亲属对照相比（分别为p = 0.016和0.043），前列腺癌患者中Tt和Aa基因型的发生率显著降低。与一级亲属相比，病例中Tt基因型的发生率显著更低（p = 0.005）。未发现与FokI多态性有显著关联。
本研究表明TaqI和ApaI多态性的杂合基因型对前列腺癌的发生具有保护作用。
