El-Attar Ahmed Z, Hussein Samia, Salama Mariam F A, Ibrahim Hanaa M, AlKaramany Amira S, Elsawi Mansour K, Hemeda Mohamed, Algazeery Ahmed
Oncology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Curr Urol. 2022 Dec;16(4):246-255. doi: 10.1097/CU9.0000000000000141. Epub 2022 Sep 16.
Prostatic epithelial cells synthesize the active form of vitamin D (1,25-dihydroxyvitamin D), which participates in regulating prostate growth. Calcitriol, a synthetic form of vitamin D, exhibits antiproliferative and prodifferentiation activities in prostate cancer. The function of 1,25-dihydroxyvitamin D is mediated by its binding to vitamin D receptor (VDR). VDR forms a heterodimer, typically with retinoid X receptor, to regulate vitamin D target genes. We evaluated the relationship between polymorphism and clinical characteristics associated with prostate cancer risk and prognosis among Egyptian men.
This case-control study included 2 groups of patients: group A, a control group of 50 subjects with benign prostate hyperplasia, and group B, 50 subjects newly diagnosed with prostate cancer. All participants performed complete blood count, liver and kidney function tests, prostate specific antigen measurement, histopathological analysis and immunohistochemistry for Dickkopf Homolog 3. Restriction fragment length polymorphism-polymerase chain reaction as performed to detect polymorphism.
Patients with prostate cancer and controls showed a significantly different genotype frequency ( = 0.007). Furthermore, prostate-specific antigen levels were significantly different in different genotypes in patients with prostate cancer ( < 0.001). Finally, T stage and the polymorphism were significantly associated ( < 0.041).
The polymorphism may be a diagnostic and prognostic marker for prostate cancer in Egyptian men.
前列腺上皮细胞合成维生素D的活性形式（1,25 - 二羟基维生素D），其参与调节前列腺生长。骨化三醇是维生素D的一种合成形式，在前列腺癌中表现出抗增殖和促分化活性。1,25 - 二羟基维生素D的功能是通过其与维生素D受体（VDR）结合来介导的。VDR通常与视黄酸X受体形成异二聚体，以调节维生素D靶基因。我们评估了埃及男性中与前列腺癌风险和预后相关的多态性与临床特征之间的关系。
这项病例对照研究包括两组患者：A组为50名良性前列腺增生患者的对照组，B组为50名新诊断为前列腺癌的患者。所有参与者均进行了全血细胞计数、肝肾功能检查、前列腺特异性抗原测量、组织病理学分析以及针对Dickkopf同源物3的免疫组织化学检测。采用限制性片段长度多态性 - 聚合酶链反应来检测多态性。
前列腺癌患者和对照组的基因型频率存在显著差异（P = 0.007）。此外，前列腺癌患者不同基因型的前列腺特异性抗原水平存在显著差异（P < 0.001）。最后，T分期与该多态性显著相关（P < 0.041）。
该多态性可能是埃及男性前列腺癌的诊断和预后标志物。
