Liti Gianni, Warringer Jonas, Blomberg Anders
IRCAN, CNRS UMR 6267, INSERM U998, University of Nice, 06107 Nice, France.
Department of Chemistry and Molecular Biology, University of Gothenburg, 40530 Gothenburg, Sweden.
Cold Spring Harb Protoc. 2017 Aug 1;2017(8):pdb.top077735. doi: 10.1101/pdb.top077735.
A small number of well-studied laboratory strains of , mostly derived from S288C, are used in yeast research. Although powerful, studies for understanding S288C do not always capture the phenotypic essence or the genetic complexity of biology. This is particularly problematic for multilocus phenotypes identified in laboratory strains because these loci have never been jointly exposed to natural selection and the corresponding phenotypes do not represent optimization for any particular purpose or environment. Isolation and sequencing of new natural yeast strains also reveal that the total sequence diversity of the global population is poorly sampled in common laboratory strains. Here we discuss methodologies required for using the natural genetic variation in yeast to complete a genotype-phenotype map.
少数经过充分研究的实验室菌株，大多源自S288C，被用于酵母研究。尽管这些研究很有影响力，但对S288C的研究并不总能捕捉到酵母生物学的表型本质或遗传复杂性。对于在实验室菌株中鉴定出的多位点表型来说，这一问题尤为突出，因为这些基因座从未共同经历过自然选择，相应的表型也不代表针对任何特定目的或环境的优化。新的天然酵母菌株的分离和测序还表明，常见实验室菌株对酵母全球种群的总序列多样性采样不足。在此，我们讨论利用酵母中的自然遗传变异来完成基因型-表型图谱所需的方法。
