CVD 石墨烯作为电化学传感平台用于生物分子的同时检测。

CVD graphene as an electrochemical sensing platform for simultaneous detection of biomolecules.

机构信息

Tianjin Key Laboratory of Film Electronic and Communicate Devices, School of Electrical and Electronic Engineering, Tianjin University of Technology, Tianjin, 300384, P.R. China.

Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin, 300384, P.R. China.

出版信息

Sci Rep. 2017 Aug 1;7(1):7044. doi: 10.1038/s41598-017-07646-2.

The development of electrochemical biosensors for the simultaneous detection of ascorbic acid (AA), dopamine (DA), uric acid (UA), tryptophan (Trp), and nitrite ([Formula: see text]) in human serum is reported in this work. Free-standing graphene nanosheets were fabricated on Ta wire using the chemical vapor deposition (CVD) method. CVD graphene, which here served as a sensing platform, provided a highly sensitive and selective option, with detection limits of AA, DA, UA, Trp, and [Formula: see text] of 1.58, 0.06, 0.09, 0.10, and 6.45 μM (S/N = 3), respectively. The high selectivity of the electrode is here explained by a relationship between the bandgap energy of analyte and the Fermi level of graphene. The high sensitivity in the oxidation current was determined by analyzing the influence of the high surface area and chemical structure of free-standing graphene nanosheets on analyte adsorption capacity. This finding strongly indicates that the CVD graphene electrode can be used as a biosensor to detect five analytes in human serum.

本文报道了一种电化学生物传感器的研制，用于同时检测人血清中的抗坏血酸（AA）、多巴胺（DA）、尿酸（UA）、色氨酸（Trp）和亚硝酸盐（[Formula: see text]）。采用化学气相沉积（CVD）法在 Ta 丝上制备了独立的石墨烯纳米片。CVD 石墨烯在这里作为传感平台，提供了高灵敏度和选择性的选择，AA、DA、UA、Trp 和 [Formula: see text]的检测限分别为 1.58、0.06、0.09、0.10 和 6.45 μM（S/N = 3）。电极的高选择性可以通过分析分析物的能带隙能量与石墨烯的费米能级之间的关系来解释。氧化电流的高灵敏度是通过分析独立石墨烯纳米片的高表面积和化学结构对分析物吸附能力的影响来确定的。这一发现强烈表明，CVD 石墨烯电极可用作生物传感器来检测人血清中的五种分析物。