Orsi Ricardo O, Santos Vivian G Dos, Pezzato Luiz E, Carvalho Pedro L P F DE, Teixeira Caroline P, Freitas Jakeline M A, Padovani Carlos R, Sartori Maria M P, Barros Margarida M
Departamento de Produção Animal, Faculdade de Medicina Veterinária e Zootecnia/FMVZ, Grupo NECTAR, Rua Prof. Doutor Walter Mauricio Correa, s/n, Caixa Postal 560, Universidade Estadual Paulista/UNESP, Campus de Botucatu, 18618-681 Botucatu, SP, Brazil.
Departamento de Melhoramento e Nutrição Animal, Faculdade de Medicina Veterinária e Zootecnia/FMVZ, Grupo AQUANUTRI, Rua Prof. Doutor Walter Mauricio Correa, s/n, Caixa Postal 560, Universidade Estadual Paulista/UNESP, Campus de Botucatu, 18618-681 Botucatu, SP, Brazil.
An Acad Bras Cienc. 2017 Jul-Sep;89(3):1785-1799. doi: 10.1590/0001-3765201720160630. Epub 2017 Jul 31.
The effect of the ethanolic extract of propolis (EEP) on Aeromonas hydrophila was analyzed by determination of minimum inhibitory concentration (MIC). Then, the effects of crude propolis powder (CPP) on growth, hemato-immune parameters of the Nile tilapia, as well as its effects on resistance to A. hydrophila challenge were investigated. The CPP (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%) was added to the diet of 280 Nile tilapia (50.0 ± 5.7 g fish-1). Hemato-immune parameters were analyzed before and after the bacterial challenge. Red blood cell, hematocrit, hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and hydrogen peroxide (H2O2) and nitric oxide (NO) were evaluated. The MIC of the EEP was 13% (v/v) with a bactericidal effect after 24 hours. Growth performance was significantly lower for those fish fed diets containing 2.5 and 3% of CPP compared to the control diet. Differences in CPP levels affected fish hemoglobin, neutrophils number and NO following the bacterial challenge. For others parameters no significant differences were observed. Our results show that although propolis has bactericidal properties in vitro, the addition of crude propolis powder to Nile tilapia extruded diets does not necessarily lead to an improvement of fish health.
通过测定最低抑菌浓度（MIC）分析了蜂胶乙醇提取物（EEP）对嗜水气单胞菌的作用。然后，研究了粗蜂胶粉（CPP）对尼罗罗非鱼生长、血液免疫参数的影响，以及其对抵抗嗜水气单胞菌攻击的作用。将CPP（0.5%、1.0%、1.5%、2.0%、2.5%和3.0%）添加到280尾尼罗罗非鱼（体重50.0±5.7克/尾）的饲料中。在细菌攻击前后分析血液免疫参数。评估了红细胞、血细胞比容、血红蛋白、平均红细胞体积（MCV）、平均红细胞血红蛋白浓度（MCHC）、过氧化氢（H2O2）和一氧化氮（NO）。EEP的MIC为13%（v/v），24小时后具有杀菌作用。与对照饲料相比，喂食含2.5%和3%CPP饲料的鱼生长性能显著较低。细菌攻击后，CPP水平的差异影响鱼的血红蛋白、中性粒细胞数量和NO。对于其他参数，未观察到显著差异。我们的结果表明，尽管蜂胶在体外具有杀菌特性，但在尼罗罗非鱼挤压饲料中添加粗蜂胶粉不一定能改善鱼的健康状况。
