Galati Luisa, Peronace Cinzia, Fiorillo Maria Teresa, Masciari Rosanna, Giraldi Cristina, Nisticò Salvatore, Minchella Pasquale, Maiolo Vincenzo, Barreca Giorgio Settimo, Marascio Nadia, Lamberti Angelo Giuseppe, Giancotti Aida, Lepore Maria Gabriella, Greco Francesca, Mauro Maria Vittoria, Borelli Annelisa, Bocchiaro Giuseppa Lo, Surace Giovanni, Liberto Maria Carla, Focà Alfredo
Institute of Clinical Microbiology, Department of Health Sciences, "Magna Graecia" University, Viale Europa, 88100 Catanzaro, Italy.
Unit of Microbiology and Virology, Polo Sanitario Nord ASP 5, Reggio Calabria, Italy.
Infect Agent Cancer. 2017 Jul 27;12:43. doi: 10.1186/s13027-017-0154-5. eCollection 2017.
Although analysis of the Human papillomavirus (HPV) genotype spread in a particular area has a crucial impact on public health and prevention programmes, there is a lack of epidemiological data regarding HPV in the Calabria region of Italy. We therefore update information on HPV age/genotype distribution by retrospectively analysing a cohort of women, with and without cervical lesions, living in Calabria, who underwent HPV DNA testing; moreover, we also evaluated HPV age/genotype distribution in a subset of patients with cervical lesions.
Cervical scrape specimens obtained from 9590 women (age range 20-75 years) from January 2010 to December 2015 were tested for HPV DNA. Viral types were genotyped by Linear Array HPV Genotyping® test (Roche, USA) at the Clinical Microbiology Operative Unit of six hospitals located in four provinces of the Calabria region. Cervical scrape specimens were also used to perform Pap smears for cytological analysis in a subset of 405 women; cytological classification of the samples was performed according to the Bethesda classification system.
A total of 2974 women (31%) (C.I. 95% 30.09-31.94) were found to be HPV DNA positive for at least one (57.3%) or several (42.7%) HPV genotypes. Of single genotype HPV infections, 46.5% and 36.4 % were classed as high-risk (HR, Group 1) and low-risk (LR, Group 3) respectively, while 16.9% were classed as probably/possibly carcinogenic and 0.2% undetermined risk. Stratified by age, total HPV distribution, showed the highest prevalence within the range 30-39 years (37.2%), while single genotype infection distribution displayed a peak in women from the age range 20-29 years (37.5%). The most common high-risk HPV type was HPV 16 (19.1%), followed by HPV 31 (9.1%).
We provide epidemiological data on HPV age/genotype distribution in women living in the Calabria region with or without cytological abnormalities, further to the enhancement of HPV screening/prevention programmes for the local population.
尽管分析人乳头瘤病毒（HPV）基因型在特定地区的传播情况对公共卫生和预防计划具有至关重要的影响，但意大利卡拉布里亚地区缺乏有关HPV的流行病学数据。因此，我们通过回顾性分析一组居住在卡拉布里亚、接受HPV DNA检测的有或无宫颈病变的女性队列，更新了HPV年龄/基因型分布的信息；此外，我们还评估了一组宫颈病变患者的HPV年龄/基因型分布情况。
对2010年1月至2015年12月期间从9590名年龄在20 - 75岁之间的女性获取的宫颈刮片标本进行HPV DNA检测。在位于卡拉布里亚地区四个省份的六家医院的临床微生物操作单元，采用线性阵列HPV基因分型检测（美国罗氏公司）对病毒类型进行基因分型。还使用宫颈刮片标本对405名女性的子集进行巴氏涂片以进行细胞学分析；样本的细胞学分类根据贝塞斯达分类系统进行。
总共发现2974名女性（31%）（95%置信区间30.09 - 31.94）至少感染一种（57.3%）或多种（42.7%）HPV基因型。在单一基因型HPV感染中，46.5%和36.4%分别被归类为高危（HR，第1组）和低危（LR，第3组），而16.9%被归类为可能/可能致癌，0.2%风险未确定。按年龄分层，总HPV分布在30 - 39岁年龄段患病率最高（37.2%），而单一基因型感染分布在20 - 29岁年龄段女性中达到峰值（37.5%）。最常见的高危HPV类型是HPV 16（19.1%），其次是HPV 31（9.1%）。
我们提供了居住在卡拉布里亚地区有或无细胞学异常女性的HPV年龄/基因型分布的流行病学数据，进一步加强了针对当地人群的HPV筛查/预防计划。
