Serrano Antonio E, Escudero Lorena V, Tebes-Cayo Cinthya, Acosta Mauricio, Encalada Olga, Fernández-Moroso Sebastián, Demergasso Cecilia
Centro de Biotecnología, Universidad Católica del Norte, Antofagasta, Chile.
Centro de Investigación Científica y Tecnológica para la Minería, Antofagasta, Chile.
Stand Genomic Sci. 2017 Jul 24;12:43. doi: 10.1186/s40793-017-0252-4. eCollection 2017.
sp 3D3 (ATCC BAA-2418) is an arsenate-reducing halotolerant strain within the phylum, isolated from the Salar de Ascotán, a hypersaline salt flat in Northern Chile. This high-Andean closed basin is an athalassohaline environment located at the bottom of a tectonic basin surrounded by mountain range, including some active volcanoes. This landscape can be an advantageous system to explore the effect of salinity on microorganisms that mediate biogeochemical reactions. Since 2000, microbial reduction of arsenic has been evidenced in the system, and the phylogenetic analysis of the original community plus the culture enrichments has revealed the predominance of phylum. Here, we describe the first whole draft genome sequence of an arsenic-reducing strain belonging to the genus showing the highest 16S rRNA gene sequence similarity (98%) with sp. strain Vns02. The draft genome consists of 57 contigs with 5,111,250 bp and an average G + C content of 37.6%. Out of 4780 total genes predicted, 4700 genes code for proteins and 80 genes for RNAs. Insights from the genome sequence and some microbiological features of the strain 3D3 are available under Bioproject accession PRJDB4973 and Biosample SAMD00055724. The release of the genome sequence of this strain could contribute to the understanding of the arsenic biogeochemistry in extreme environments.
sp 3D3（ATCC BAA - 2418）是该菌门内一种能还原砷的耐盐菌株，从智利北部的阿斯科特án盐沼（一个高盐度盐滩）分离得到。这个位于安第斯山脉高处的封闭盆地是一个无海相卤水环境，位于一个被山脉环绕的构造盆地底部，其中包括一些活火山。这种地貌可能是一个有利的系统，可用于探索盐度对介导生物地球化学反应的微生物的影响。自2000年以来，该系统中已证实存在微生物对砷的还原作用，对原始群落以及培养富集物的系统发育分析揭示了该菌门的优势地位。在此，我们描述了属于该属的一种砷还原菌株的首个全基因组草图序列，该菌株与sp.菌株Vns02的16S rRNA基因序列相似度最高（98%）。基因组草图由57个重叠群组成，长度为5,111,250 bp，平均G + C含量为37.6%。在总共预测的4780个基因中，4700个基因编码蛋白质，80个基因编码RNA。菌株3D3的基因组序列见解以及一些微生物学特征可在生物项目登录号PRJDB4973和生物样本SAMD00055724下获取。该菌株基因组序列的发布可能有助于理解极端环境中的砷生物地球化学。
