Brioukhanov Andrei L, Kadnikov Vitaly V, Beletsky Alexey V, Savvichev Alexander S
Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.
Skryabin Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, Russia.
Microorganisms. 2023 Jun 23;11(7):1642. doi: 10.3390/microorganisms11071642.
The strain WBS, an anaerobic, psychro- and halotolerant bacterium belonging to the genus , was isolated from the littoral bottom sediments of the White Sea, Arctic, Russia. WBS grew at temperatures between 8 and 32 °C (optimum growth at 18-20 °C), pH between 5.2 and 8.3 (optimum growth at pH 7.2), and at NaCl concentrations between 0 and 70 g L (optimum growth at 32 g L). It reduced sulfate, thiosulfate, and elemental sulfur into sulfide, and, probably, the strain is able to disproportionate thiosulfate. The strain also utilized a wide range of substrates as it is a chemoorganotrophic bacterium. Analysis of the sequenced genome revealed genes for all enzymes involved in the Embden-Meyerhof glycolytic pathway as well as genes for the non-oxidative stage of the pentose phosphate pathway. The presence of genes encoding aldehyde dehydrogenases and alcohol dehydrogenases also suggests that, in addition to acetate, alcohols can also be the fermentation products. The strain possessed superoxide dismutase and peroxidase activities and the ability to consume O, which is in full accordance with the presence of corresponding genes of antioxidant defense in the genome. The phylogenetic analysis suggested that the strain WBS is the closest relative of LTF Kr01 (16S rRNA gene sequence similarity 98.78%). Based on biochemical and genomic characteristics, the strain WBS is proposed to represent a novel aero-, halo- and psychrotolerant strain from the genus , isolated for the first time among its members from cold oxygenated marine bottom sediments.
菌株WBS是一种厌氧、耐冷和耐盐细菌，属于 属，从俄罗斯北极白海的沿岸底部沉积物中分离得到。WBS在8至32°C的温度下生长（最适生长温度为18 - 20°C），pH值在5.2至8.3之间（最适生长pH值为7.2），NaCl浓度在0至70 g/L之间（最适生长浓度为32 g/L）。它能将硫酸盐、硫代硫酸盐和元素硫还原为硫化物，并且该菌株可能能够使硫代硫酸盐发生歧化反应。由于该菌株是一种化能有机营养细菌，它还能利用多种底物。对测序基因组的分析揭示了参与Embden - Meyerhof糖酵解途径的所有酶的基因以及戊糖磷酸途径非氧化阶段的基因。编码醛脱氢酶和醇脱氢酶的基因的存在也表明，除了乙酸盐外，醇类也可能是发酵产物。该菌株具有超氧化物歧化酶和过氧化物酶活性以及消耗O的能力，这与基因组中抗氧化防御相应基因的存在完全一致。系统发育分析表明，菌株WBS是LTF Kr01的最亲近亲缘种（16S rRNA基因序列相似性为98.78%）。基于生化和基因组特征，菌株WBS被提议代表 属中一种新的需氧、耐盐和耐冷菌株，首次从其成员中从寒冷的含氧海洋底部沉积物中分离得到。
