Biological Physics Laboratory, Institute of Physics, Universidad Autónoma de San Luis Potosí, Álvaro Obregón 64, San Luis Potosí, S. L. P. 78000, Mexico.
Nanoscale. 2017 Aug 17;9(32):11625-11631. doi: 10.1039/c7nr02118f.
The design and construction of novel nanocarriers that have controlled shape and size and are made of inherently biocompatible components represents a milestone in the field of nanomedicine. Here, we show the tailoring of nanoliposphere-like particles for use as biocompatible drug nanocarriers. They are made with the building block components present in human lipoproteins by means of microfluidization, which allows for good size and polydispersity control, mimicking the physical properties of natural low-density lipoproteins (LDLs). This new type of nanocarrier has a negative surface charge and a hydrophobic core that allow the stabilization and encapsulation of hydrophobic anticancer drugs such as camptothecin, resulting in anticancer drug-loaded nanolipospheres. However, we found that the nanoparticles are unstable since their size increases with time. These nanolipospheres were further encapsidated using the non-cytotoxic capsid protein of the plant virus CCMV, which renders the nanoparticles stable. In a more general application, this new virus-like particle confers a controlled microenvironment for the transport of any kind of hydrophobic drug that can bypass the cellular defense mechanisms and deliver its payload.
新型纳米载体的设计和构建，其具有可控的形状和大小，并且由固有生物相容性的组成部分制成，这代表了纳米医学领域的一个里程碑。在这里，我们展示了纳米脂质体样颗粒的定制，用作生物相容性药物纳米载体。它们是通过微流技术用存在于人类脂蛋白中的构建块成分制成的，这允许很好地控制尺寸和多分散性，模拟天然低密度脂蛋白（LDL）的物理性质。这种新型纳米载体具有负表面电荷和疏水性核心，允许稳定和封装疏水性抗癌药物，如喜树碱，从而得到载有抗癌药物的纳米脂质体。然而，我们发现纳米颗粒不稳定，因为它们的尺寸随时间增加。通过使用植物病毒 CCMV 的非细胞毒性衣壳蛋白进一步封装这些纳米脂质体，从而使纳米颗粒稳定。在更一般的应用中，这种新型病毒样颗粒为任何类型的疏水性药物的运输提供了一个可控的微环境，这些药物可以绕过细胞防御机制并传递其有效载荷。
